文章摘要
蒋 理,徐 建,杨瑞霞,谢而付.自身抗体IgG检测蛋白芯片检测抗ENA抗体谱的研究[J].南京医科大学学报,2009,29(3):382~385
自身抗体IgG检测蛋白芯片检测抗ENA抗体谱的研究
Study on detection of anti-ENA antoantibody profiles with IgG autoantibody assay protein chip
投稿时间:2008-09-19  
DOI:10.7655
中文关键词: 蛋白芯片  ENA抗体谱  免疫印迹
英文关键词: protein chip  anti-ENA autoantibody profiles  immunoblotting test
基金项目:
作者单位
蒋 理 南京医科大学第一附属医院医学检验科,江苏 南京 210029 
徐 建  
杨瑞霞  
谢而付  
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中文摘要:
      目的:使用蛋白芯片技术检测患者血清中的抗ENA抗体谱,评价蛋白芯片技术检测抗ENA抗体谱的性能?方法:收集249例患者血清,用自身抗体IgG检测蛋白芯片进行抗ENA抗体谱检测,应用PBT型生物芯片识别系统进行图像分析,以免疫印迹方法作为对照?结果:自身抗体IgG检测蛋白芯片与免疫印迹法方法相比,符合率分别为:抗SS-A/Ro抗体82.2%?抗SS-B/La抗体88.2%?抗Scl-70抗体94.7%?抗Jo-1抗体95.9%?抗Sm抗体92.5%?抗u1RNP抗体88.5%?抗Rib-P抗体82.2%?结论:自身抗体IgG检测蛋白芯片可应用于抗ENA抗体谱检测,与免疫印迹技术相比,检测结果无显著性差异?
英文摘要:
      Objective:To evaluate the property of IgG autoantibody assay protein chip in detecting anti-ENA autoantibody profiles. Methods:The sera of 249 patients were collected. The samples were detected with IgG autoantibody assay protein chip in comparison with immuloblotting test. Results:IgG autoantibody assay protein chip can detect anti-ENA autoantibody profiles in serum of patients. In comparison with immunoblotting assay, the coincidence rate was 82.2% for anti-SS-A/Ro, 88.2% for anti-SS-B/La, 94.7% for anti-Scl-70, 95.9% for anti-Jo-1, 92.5% for anti-Sm, 88.5% for anti-ulRNP, 82.2% for anti-Rib-P, respectively. Conclusion:IgG autoantibody assay protein chip can be used to detect anti-ENA autoantibody profiles from patients serum. There is no significant difference between detection results from IgG autoantibody assay protein chip and that from immunblotting test.
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