Objective:To explore the effects of Ambrolic acid(AmbA) on proliferation and apoptosis of human gastric cancer SGC-7901 cell line. Methods:MTT assay was used to observe the anti-proliferation effect of AmbA on SGC-7901 cells with morphological changes observed under optical microscopy at the same time. The apoptosis rates and cell cycle status of SGC-7901 cells treated by AmbA were determined by flow cytometry(FCM). Results:AmbA inhibited the proliferation of SGC-7901 cells in a dose and time-dependent manner. FCM analysis showed that cells were arrested in S phase and induced apoptosis dose-dependently. Conclusion: AmbA has significant antitumor activity in vitro. The potential mechanism of anti-proliferation effect caused by AmbA may be cell-cycle arrest in S phase and cell apoptosis.