文章摘要
蒋 慧,白小明,冷 静.PGE2对HuH7细胞Survivin表达影响的研究[J].南京医科大学学报,2009,29(5):589~593
PGE2对HuH7细胞Survivin表达影响的研究
The effect of PGE2 on the expression of Survivin in hepatocellular carcinoma
投稿时间:2009-12-29  
DOI:10.7655
中文关键词: 肝细胞癌  PGE2  EP受体  Survivin  PI3K/AKT
英文关键词: hepatocellular carcinoma  PGE2  EP receptor  Survivin  PI3K/AKT
基金项目:国家自然科学基金资助(30470784,30871015)
作者单位
蒋 慧 南京医科大学生殖医学重点实验室,肿瘤中心,病理学系,江苏 南京 210029 
白小明  
冷 静  
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中文摘要:
      目的:研究前列腺素E2(PGE2)对人肝癌细胞HuH7 Survivin表达的影响及其与PI3K/AKT信号转导通路的相关性?方法:用PGE2?EP受体激动剂?EP受体抑制剂?PI3K抑制剂(LY294002)处理HuH7细胞,用Western blot检测Survivin蛋白表达?结果:5 μmol/L PGE2处理HuH7细胞8 h后,Survivin的表达水平与对照组相比上升了63.59%(P < 0.01);5 μmol/L EP1?5 μmol/L EP3?5 μmol/L EP4受体激动剂分别处理HuH7细胞8 h后,Survivin的表达水平与对照组相比分别上升了114.76%(P < 0.01),76.68%(P < 0.01),70.01%(P < 0.01),而5 μmol/L EP2受体激动剂处理HuH7细胞8 h后,Survivin的表达水平与对照组相比无明显改变(P > 0.05);10 μmol/L EP1,10 μmol/L EP4受体抑制剂分别处理HuH7细胞8 h后,Survivin的表达水平与PGE2组相比分别下降了32.95%(P < 0.01),28.36%(P < 0.05),而10 μmol/L EP2受体抑制剂处理HuH7细胞8 h后,Survivin的表达水平与PGE2组相比无明显改变(P > 0.05);10 μmol/L PI3K抑制剂(LY294002)处理HuH7细胞8 h后,Survivin的表达水平与PGE2组相比下降了28.05%(P < 0.01)?结论:PGE2可能通过EP1?EP3?EP4这3种受体影响HuH7细胞中Survivin的表达,且这种调节作用可能与PI3K/Akt信号转导通路有关?
英文摘要:
      Objective:To investigate the effect of PGE2 on the expression of Survivin in HuH7 cell line and its correlation with PI3K/AKT signal conduction pathway. Methods:HuH7 cells were treated with PGE2,Celecoxib(EP receptor agonist),LY294002(EP receptor inhibitor). Western blotting was employed to detect the expression of Survivin in HuH7 cells. Results:The expression of Survivin in HuH7 cells were increased by 63.59%,114.76%,76.68% and 70.01%(P < 0.01)after treatment of PGE2(5 μmol/L) and EP1,EP3,EP4 receptor agonist(5 μmol/L)for 8 h,and decreased by 32.95%(P < 0.01)and 28.36%(P < 0.05) after treatment of EP1 and EP4 receptor inhibitor(10 μmol/L)for 8 h respectively,but have no change after treatment of EP2 receptor agonist(5 μmol/L) and EP2 receptor inhibitor(5 μmol/L)(P > 0.05). After the treatment of LY294002(10 μmol/L) for 8 h,the expression of Survivin in HuH7 cells were decreased by 28.05%(P < 0.01) vs that treated with PGE2 . Conclusion:Our findings suggested that PGE2 might up-regulate the expression of Survivin through EP1,EP3 and EP4 receptors,which could be partly related to the PI3K/AKT signal conduction pathway.
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