文章摘要
刘静宁,顾 振,苗登顺.甲状旁腺素在胸腺的表达及血钙对其表达的调节[J].南京医科大学学报,2009,29(5):634~639
甲状旁腺素在胸腺的表达及血钙对其表达的调节
Expression of parathyroid hormone in mouse thymus and its regulation by serum calcium
投稿时间:2009-01-10  
DOI:10.7655
中文关键词: 胸腺  PTH  血钙  基因敲除
英文关键词: thymus  PTH  calcium  gene knock-out
基金项目:江苏省卫生厅基金资助(H200627)
作者单位
刘静宁 南京医科大学人体解剖学系,江苏 南京 210029 
顾 振  
苗登顺  
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中文摘要:
      目的:为了证实PTH基因在胸腺的表达,明确PTH蛋白在胸腺组织的定位以及胸腺来源的PTH是否受到血钙水平改变的调节?方法:使用RT-PCR?real-time PCR和免疫组织化学的方法比较分析同窝2周龄PTH基因敲除纯合子(PTH-/-)和野生型(WT)小鼠以及1-α 羟化酶基因敲除纯合子[1α(OH)ase-/-]和野生型(WT)小鼠的甲状旁腺和胸腺的PTH表达差异?结果:PTH-/-小鼠的甲状旁腺和胸腺无PTH表达;同窝WT小鼠甲状旁腺和胸腺均有PTH表达,但胸腺表达水平较甲状旁腺低?PTH在胸腺上皮细胞表达,而不在胸腺淋巴细胞表达?1α(OH)ase-/-小鼠表现为低钙血症?与正常血钙的WT小鼠相比,PTH基因和蛋白的表达水平在1α(OH)ase-/-小鼠胸腺组织中均明显升高?结论:胸腺组织不仅是产生PTH的辅助器官,而且其PTH表达也受到血钙水平的调节?
英文摘要:
      Objective:To confirm the expression of parathyroid hormone(PTH) gene in mouse thymus,determine the localization of PTH protein in mouse thymus and explore whether thymus-derived PTH is regulated by the alteration of serum calcium levels. Methods:The difference of PTH gene and protein expression in parathyroid gland and thymus were compared between PTH knock-out mice 1α(OH)ase knock-out mice and their wild-type littermates at 2 weeks of ages by RT-PCR,real-time PCR and immunohistohemistry. Results:PTH gene and protein were undetectable in parathyroid gland and thymus in PTH knock-out mice,but were detectable in both organs in wild-type mice,despite the fact that the gene and protein expression of PTH was lower in the thymus than in the parathyroid glands. PTH was localized in the epithelium,but not in lymphocytes in thymus. PTH gene and protein expression levels were up-regulated in parathyroid gland and thymus in 1α(OH)ase knock-out mice with hypocalcaemia. Conclusion:The thymus is the another source of parathyroid hormone,in which the expression of PTH is regulated by the alteration of serum calcium levels.
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