文章摘要
吴 瑞,王 青,范卫民,任科伟.阿仑膦酸钠对人工关节磨损微粒刺激破骨细胞分化形成的影响[J].南京医科大学学报,2009,29(5):686~690
阿仑膦酸钠对人工关节磨损微粒刺激破骨细胞分化形成的影响
Effect of alendronate on osteoclastogenesis induced by wear debris
投稿时间:2008-12-23  
DOI:10.7655
中文关键词: 破骨细胞  二磷酸盐  磨损微粒  分化
英文关键词: osteoclast  bisphosphonate  wear debris  differentiation
基金项目:江苏省卫生厅科技发展基金资助(H200601)
作者单位
吴 瑞 南京医科大学第一附属医院骨科,江苏 南京 210029 
王 青  
范卫民  
任科伟  
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中文摘要:
      目的:观察二磷酸盐类药物阿仑膦酸钠对人工关节磨损微粒刺激破骨细胞分化形成的影响,探讨二磷酸盐防治人工关节无菌性松动的可能机制?方法:体外用巨噬细胞集落刺激因子(M-CSF)诱导小鼠骨髓造血干细胞分化为破骨前体细胞(osteoclast precursors,OCPs),然后用核因子-κB受体活化因子配体(RANKL)和M-CSF两种细胞因子协同诱导OCPs向成熟破骨细胞分化,同时在培养体系中加入高分子聚乙烯微粒(103/ml)和不同浓度的阿仑膦酸钠(0.4?2.0?10.0?50.0 ?滋g/ml),对获得的各组破骨细胞行抗酒石酸酸性磷酸酶(TRAP)染色计数阳性破骨细胞(核 ≥ 3)的数目;提取总RNA用逆转录-聚合酶链反应(RT-PCR)检测TRAP mRNA的表达?结果:高分子聚乙烯微粒组TRAP阳性破骨细胞数目和TRAP mRNA的表达显著高于空白对照组(P < 0.05);阿仑膦酸钠组TRAP阳性破骨细胞数目和TRAP mRNA的表达均显著低于高分子聚乙烯组(P < 0.05),且随着阿仑膦酸钠浓度增高TRAP阳性破骨细胞数目和TRAP mRNA的表达呈递减趋势,组间差异均有统计学意义(P < 0.05)?结论:阿仑膦酸钠可以抑制人工关节磨损微粒刺激破骨细胞分化形成?
英文摘要:
      Objective:To explore the mechanism of alendronate on the formation of osteoclast from osteoclast precursors(OCPs) induced by wear debris,and provide more evidences for bisphosphonate on the treatment of aseptic loosening of prosthesis. Methods:OCPs were induced from hematopoietic stem cells in the presence of macrophage colony stimulating factor(M-CSF), and then OCPs were induced into osteoclast cells in the presence of M-CSF and receptor activator of NF-κB ligand(RANKL), and then polyethylene(103/ml) and different concentration alendronate(0.4 ?滋g/ml,2.0 ?滋g/ml,10.0 ?滋g/ml,50.0 ?滋g/ml) were added to the culture medium. After cultured for 4 days,the cells were stained with TRAP. Multinucleated( ≥ 3 nuclei) TRAP-positive cells were counted as osteoclasts, and the expression of TRAP mRNA were determined by RT-PCR. Results:The number of TRAP+ osteoclasts and the expression of TRAP mRNA from the polyethylene group were significantly higher than those in control group(P < 0.05), which in alendronate groups were all lower than those in the polyethylene group,and the number of TRAP+ osteoclasts and the expression of TRAP were decreased with the concentration increase of alendronate(P < 0.05). Conclusion:Bisphosphonate can inhibit the formation of osteoclast from osteoclast precursors induced by wear debris.
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