文章摘要
沈 红,解卫平,王 虹,金 宇,蔡健康.埃他卡林对低氧性肺动脉高压大鼠内皮素受体mRNA表达的影响[J].南京医科大学学报,2009,29(5):691~694
埃他卡林对低氧性肺动脉高压大鼠内皮素受体mRNA表达的影响
Effects of Lptakalim on mRNA expression of endothelin receptors in rats with hypoxic pulmonary hypertension
投稿时间:2009-01-13  
DOI:10.7655
中文关键词: 埃他卡林  低氧性肺动脉高压  内皮素受体  ATP敏感性钾通道  RT-PCR
英文关键词: Iptakalim  hypoxic pulmonary hypertension  endothelin receptor  ATP sensitive potassium channel  RT-PCR
基金项目:南京医科大学科技发展基金面上项目(06NMUM031);江苏省自然科学基金(BK2006246)
作者单位
沈 红 南京医科大学第二附属医院呼吸内科,江苏 南京 210011 
解卫平 南京医科大学第一附属医院呼吸内科,江苏 南京 210029 
王 虹 南京医科大学第一附属医院呼吸内科,江苏 南京 210029 
金 宇 南京医科大学第一附属医院呼吸内科,江苏 南京 210029 
蔡健康 南京医科大学第二附属医院呼吸内科,江苏 南京 210011 
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中文摘要:
      目的:研究新型ATP敏感性钾通道开放剂埃他卡林(IPT)对低氧性肺动脉高压(HPH)大鼠内皮素A受体(ETAR)?内皮素B受体(ETBR)mRNA表达的影响?方法:SD大鼠平均分成正常对照组?HPH组?IPT处理组(对每天缺氧饲养的大鼠给予IPT 1.5 mg/kg灌胃),后两组置于常压低氧舱中每天8 h,每周6天,4周后逆转录多聚酶联式反应(RT-PCR)方法检测肺组织ETAR和ETBR mRNA表达?结果:与正常对照组相比,HPH组大鼠ETAR mRNA表达水平明显上升(P < 0.01),ETBR mRNA表达水平明显下降(P < 0.01);与HPH组相比,埃他卡林干预的缺氧大鼠ETAR mRNA表达明显降低(P < 0.01),ETBR mRNA表达明显增加(P < 0.01)?结论:HPH模型大鼠肺组织内皮素受体mRNA表达发生异常,而埃他卡林可部分逆转这种异常,有助于抑制HPH血管收缩和平滑肌增殖?
英文摘要:
      Objective:To investigate the effects of Iptakalim(IPT),a novel ATP sensitive potassium channel opener,on the lung mRNA expression of endothelin A receptor(ETAR)and endothelin B receptor(ETBR)in hypoxic pulmonary hypertension(HPH). Methods:SD rats were equally divided into normal-controlled group,HPH-model group,and IPT treated group(treated with IPT 1.5 mg/(kg·d) prior to hypoxia). The HPH-model group and IPT treated group were bred in normal baric and hypoxic condition,8 hours a day,6 days a week,for 4 weeks. Reverse transcription polymerase chain reaction(RT-PCR)was performed to analyze the expression of ETAR and ETBR. Results:The mRNA expression of ETAR was markedly higher(P < 0.01),whereas the level of ETBR mRNA was significantly lower in HPH rats than in control(P < 0.01);Iptakalim significantly inhibited the expression of ETAR mRNA(P < 0.01),and enhanced the expression of ETBR in HPH rats(P < 0.01). Conclusion:There were significantly alterations in gene expression of ET receptors in the lung of HPH,and Iptakalim could partly reverse this expression,which might contribute to inhibit vasoconstriction and vascular proliferation.
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