Objective:To analyze the differential expression of proteome between metastatic lymph nodes in adenocarcinoma of lung and normal lymph nodes. Methods:The proteome techniques were used to separate the protein spots and analyze the differential expression of proteome in the metastatic lymph nodes and normal lymph nodes,and verification was performed at protein and mRNA level,Results:Compared with control,there were 23 protein spots significantly differentially expressed,of which 4 protein spots were newly found,1 protein spot disappeared,14 protein spots increased markedly,and 4 protein spots decreased significantly 11 kinds of the associated protein were identified,among them,ANX1,CK18,Rho-GDP,GDIR,TPMF,TGLC and IL-18 were down-regulated expression significantly in metastatic lymph nodes,but CLI1,ER60,CK,PDX and CH60 were up-regulated expression significantly. Conclusion:The proteomic expression in metastatic lymph nodes of adenocarcinoma of lung is significantly different compared with of the normal control,the 11 kinds of proteins may be associated with the mechanism of metastasis of adenocarcinoma of lung.