Objective:To study the biocompatibility and mechanical properties of PLGA membrane modified by Type-Ⅰcollagen and Chitosan,and to develop a novel tissue engineering materials as artificial spinal dura mater. Methods:PLGA membrane(membraneⅠ),PLGA/ Type-Ⅰcollagen composite membrane(membraneⅡ),PLGA/ Type-Ⅰcollagen/ Chitosan(9 ∶ 1) composite membrane(membrane Ⅲ) and PLGA/ Type-Ⅰcollagen/ Chitosan(5 ∶ 5) composite(membrane Ⅳ) were produced through a certain process. Contact angle,absorption rate,cytotoxicity study and determination of mechanical properties were used to research all type membranes. Results:Contact angle:membraneⅡ < membraneⅢ < membraneⅣ < membraneⅠ,P < 0.01; Absorption rate:membraneⅠ < membrane Ⅳ < membrane Ⅲ < membrane Ⅱ,P < 0.01;Cytotoxic experiment(MTT method):at the 1st day,the OD value between each group didn’t have significant difference,P > 0.05. At the 3rd and the 7th day,there was significant difference between membrane Ⅰand Ⅱor Ⅲ,membrane Ⅲ and Ⅳ,P < 0.05. After being modified by Type-Ⅰcollagen and Chitosan, PLGA membrane striking enhanced the adhesion and proliferation of L929 cell,and the comparison between all type membranes showed that there was significant difference between membrane Ⅰand other type membranes,P < 0.05,and there was no significant difference between membrane Ⅱ,Ⅲ and Ⅳ. Conclusion:With type-Ⅰcollagen and Chitosan(9 ∶ 1) appending to the exterior, PLGA can enhance the biocompatibility and Mechanical properties of membrane.