碱性成纤维细胞生长因子对肺癌A459细胞pim-3表达影响的实验研究
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Effect of basic fibroblast growth factor on pim-3 in humanl lung cancer cell A459
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    摘要:

    目的:探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,FGF-2)对肺癌A459细胞株pim-3的影响-方法:不同浓度的(0-25-50-100 ng/ml)FGF-2在体外作用于肺癌A459细胞,半定量逆转录聚合酶链式反应(RT-PCR)法检测pim-3 mRNA,Western blot检测pim-3蛋白表达-结果:与空白对照组比较,RT-PCR 和Western blot检测结果显示pim-3 mRNA及其蛋白的表达在各FGF-2作用组均显著增高,各组间比较差异有统计学意义(P < 0.01)-结论:FGF-2可以量效的方式上调pim-3 的表达-

    Abstract:

    Objective:To investigate the effect of basic fibroblast growth factor(FGF-2) on pim-3 in humanl lung cancer cell A459. Methods:Lung cancer cell A459 was treated by different concentrations(0-25-50-100 ng/mL) of FGF-2,pim-3 mRNA was measured by reverse transcription polymerase chain reaction(RT-PCR),and western blotting was used to detect the expression of PIM-3 protein. Results:The expressions of pim-3 mRNA and protein increased dramaticly in the groups treated with FGF-2 compared with conorol,and there was a significantly difference between each two experiment groups(P < 0.01). Conclusion:FGF-2 can significantly upregulate the expression of pim-3 in dosage-dependent manner.

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贾晓民,赵 杰,王海清.碱性成纤维细胞生长因子对肺癌A459细胞pim-3表达影响的实验研究[J].南京医科大学学报(自然科学版),2009,29(6):845-847

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  • 收稿日期:2009-12-22
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