文章摘要
贾晓民,赵 杰,王海清.碱性成纤维细胞生长因子对肺癌A459细胞pim-3表达影响的实验研究[J].南京医科大学学报,2009,29(6):845~847
碱性成纤维细胞生长因子对肺癌A459细胞pim-3表达影响的实验研究
Effect of basic fibroblast growth factor on pim-3 in humanl lung cancer cell A459
投稿时间:2009-12-22  
DOI:10.7655
中文关键词: 碱性成纤维细胞生长因子  肺癌  pim-3
英文关键词: FGF-2  lung cancer  pim-3
基金项目:
作者单位
贾晓民 徐州医学院第二附属医院呼吸内科,江苏 徐州 221006 
赵 杰  
王海清  
摘要点击次数: 1563
全文下载次数: 188
中文摘要:
      目的:探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,FGF-2)对肺癌A459细胞株pim-3的影响?方法:不同浓度的(0?25?50?100 ng/ml)FGF-2在体外作用于肺癌A459细胞,半定量逆转录聚合酶链式反应(RT-PCR)法检测pim-3 mRNA,Western blot检测pim-3蛋白表达?结果:与空白对照组比较,RT-PCR 和Western blot检测结果显示pim-3 mRNA及其蛋白的表达在各FGF-2作用组均显著增高,各组间比较差异有统计学意义(P < 0.01)?结论:FGF-2可以量效的方式上调pim-3 的表达?
英文摘要:
      Objective:To investigate the effect of basic fibroblast growth factor(FGF-2) on pim-3 in humanl lung cancer cell A459. Methods:Lung cancer cell A459 was treated by different concentrations(0?25?50?100 ng/mL) of FGF-2,pim-3 mRNA was measured by reverse transcription polymerase chain reaction(RT-PCR),and western blotting was used to detect the expression of PIM-3 protein. Results:The expressions of pim-3 mRNA and protein increased dramaticly in the groups treated with FGF-2 compared with conorol,and there was a significantly difference between each two experiment groups(P < 0.01). Conclusion:FGF-2 can significantly upregulate the expression of pim-3 in dosage-dependent manner.
查看全文   查看/发表评论  下载PDF阅读器
关闭