Objective:To investigate the effect of active immunization against gastric inhibitory popeptide(GIP)on behavior of SD rats. Methods:Male rats were fed with high-fat diet and divided into control group and GIP immunized group. Animals of experimental group and control group were immunized subcutaneously with 400 -滋g of GIP-KLH and KLH at the indicated time points respectively. GIP-specific antibody titers were determined by ELISA according to standard protocols differences on GIP-induced the insulin secretion of MIN6 cells using GIP-specific antibodies and behavior between two groups were investigated. Results:After four weeks of single immunization,high GIP-specific antibody titers were induced. Antibody levels further increased with continue injections. Compared with the control group,GIP-KLH immunized rats’serum containing specific antibodies significantly inhibited GIP-induced the insulin secretion on MIN6 cells(P < 0.01).Compared with control rats,distance(P < 0.01),speed(P < 0.01),active times(P < 0.05),the number of activities(P < 0.01)in spontaneous activity test were different(P < 0.05)significantly in GIP immunised group. Both the two groups showed no significant differences in test section of Morris water maze test(P > 0.05). Conclusion:Active immunization against GIP resulted in high,GIP-specific antibody titers. GIP immunized rats had a decreasing spontaneous activity.