Objective:To investigate the expressions of survivin,caspase-3 and NF-κB in hepatocellular carcinoma(HCC)and their relationship with clinical pathologic features of HCC. Methods:40 cases of HCC specimens were collected as study group and 10 cases of normal specimens as control. Immunohistochemcal method was used to detect the expressions of survivin,caspase-3 and NF-κB. Results:Positive expression rate of survivin was 67.5% in HCC study group,while negative in the control. There was significant difference between the study and normal control groups(-字2=12.082,P < 0.05). In the study group,the positive expression rate of survivin was higher in the group with oncogenic portal vein thrombosis than in the group without(-字2=5.405,P < 0.05). The positive rate of survivin was higher in the cases with extrahepatic metastasis than in coses without(-字2=4.596,P < 0.05). 35.0% of the study group expressed caspase-3,while 80.0% of the control group expressed caspase-3. Significant difference was found between these two groups(-字2=4.875,P < 0.05). 77.5% of the experimental group expressed NF-κB while negative in the control. There was significant difference between these two groups(-字2=17.238,P < 0.05). The positive expression rate of NF-κB was higher in the low differentiation group than those in the high differentiation group(-字2=4.198,P < 0.05). There was negative correlation between the expression of caspase-3 and survivin(r=-0.679,P < 0.05). However,the correlation between the expression of NF-κB and survivin was positive(r=0.519,P < 0.05). Conclusion:Since survivin,caspase-3 and NF-κB each has a different effect on the origin and development of HCCs,the combination of detection of these three factors will provide us a valuable clinical index for the degree of malignancy and capability of metastasis,it offers HCC a better treatment regimen and prognosis.