文章摘要
王 涛,黄智敏,俞香宝,孙 强,钱 军,赵秀芬,邢昌赢.不同病理类型肾小球疾病患者血清中IL-4?IL-10和IFN-γ的改变及其临床意义[J].南京医科大学学报,2009,29(8):1138~1141
不同病理类型肾小球疾病患者血清中IL-4?IL-10和IFN-γ的改变及其临床意义
Changes of serum interleukin(IL)-4,IL-10 and interferon(IFN)-?酌 in patients with different pathological types of primary glomerular disease and its clinical significance
投稿时间:2009-04-07  
DOI:10.7655
中文关键词: 肾小球疾病  病理  细胞因子  ELISA
英文关键词: glomerular disease  pathology  cytokine  ELISA
基金项目:“十一五”国家科技支撑项目(2007BAI20B102)
作者单位
王 涛 南京医科大学第一附属医院肾内科,江苏 南京 210029 
黄智敏  
俞香宝  
孙 强  
钱 军  
赵秀芬  
邢昌赢  
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中文摘要:
      目的:探讨不同病理类型肾小球疾病患者血清中白介素(interleukin,IL)-4?IL-10和干扰素(interferon,IFN)-?酌水平的变化及其临床意义?方法:采集12例局灶节段性肾小球硬化(FSGS)?12例局灶增生性肾小球肾炎(FPGN)?12例系膜增生性肾小球肾炎(MsPGN)?12例膜性肾病(MN)?12例狼疮性肾炎(LN)以及12例正常人的静脉血,采用双抗体夹心ELISA法检测血清中的IL-4?IL-10和IFN-?酌水平?结果:MsPGN组?MN组和FPGN组的IL-4?IL-10有所上调,而IFN-?酌则有所下调;FSGS组IL-4?IL-10明显下调,IFN-?酌有上调;而LN组IFN-?酌有显著上调,IL-4?IL-10稍上调,并且IL-4水平与C反应蛋白水平呈负相关,与补体C3水平正相关?结论:MsPGN?MN?FPGN组患者发生Th2细胞主导的炎症,FSGS组患者发生Th1细胞主导的炎症,LN患者处于Th1/Th2高水平的相互作用,而以Th1细胞主导的炎症病变中,可以通过细胞因子的测定结合临床指标初步判断肾小球疾病患者病理类型?
英文摘要:
      Objective:To study the changes of serum IL-4, IL-10 and IFN-γ in patients with different pathological types of primary glomerular disease and its clinical significance. Methods:Twelve cases with FSGS, 12 cases with focal proliferative glomerulonephritis, 12 cases with MsPGN, 12 cases with MN, 12 cases with lupus nephritis and 12 normal controls were involved in this experiment,and their serum levels of IL-4,IL-10,IFN-γ were measured with sandwich ELISA. Results:The levels of IL-4 and IL-10 in MsPGN, MN, FPGN increased, while the level of IFN-γ in them decreased. The level of serum IL-4 and IL-10 in FSGS decreased, while the IFN-γ level increased. The IFN-γ level in LN highly increased,and the IL-4 and IL-10 level increased a little. And results showed that there was negative-correlation of IL-4 level with serum C-reactive protein, and positive-correlation with serum C3 level. Conclusion:The Th2 cells play a dominant role in patients with MsPGN, MN and FPGN, while the Th1 cells play a dominant role in patients with FSGS; there is a high-level interaction between Th1 and Th2 in lupus nephritis patients, and the Th2 cells play a dominant role. The pathological kinds of primary glomerular disease could be initially detected by measuring the level of IL-4, IL-10 and IFN-γ, combining with some other clinical characteristics.
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