文章摘要
孙国荣,丁友成,张 辉,周主青,钟明安,赵中辛.大肠癌肝转移灶及其周围正常肝组织中mmp2表达的比较[J].南京医科大学学报,2009,29(9):1229~1231
大肠癌肝转移灶及其周围正常肝组织中mmp2表达的比较
The comparison of mmp2 expression of hepatic metastases and surrounding normal liver tissues in colorectal cancer with liver metastasis
投稿时间:2009-04-20  
DOI:10.7655
中文关键词: 结肠直肠肿瘤  肝脏转移  mmp2
英文关键词: colorectal cancer  hepatic metastasis  mmp2
基金项目:
作者单位
孙国荣 上海同济大学附属东方医院普外科,上海 200120 
丁友成  
张 辉  
周主青  
钟明安  
赵中辛  
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中文摘要:
      目的:探讨mmp2基因在大肠癌肝脏转移中的意义?方法:收集手术切除21例大肠癌合并肝脏转移患者的原发病灶?癌旁正常黏膜组织?肝转移病灶及其周边正常肝组织的新鲜组织标本,用实时定量rt-pcr法测定各标本的mmp2 mrna表达,并用western blot方法检测其蛋白表达?结果:在肝转移灶?肝转移灶周边正常肝组织?大肠癌原发灶和正常大肠黏膜之间,全部病例的mmp2 mrna表达均有明显差异,但以肝转移灶表达最高,正常大肠粘膜表达最低?其中18例在正常大肠黏膜中的mmp2蛋白表达低于肝转移灶?肝转移灶周边正常肝组织和原发灶?而所有病例原发灶与肝转移灶中的mmp2蛋白表达无差异,并且这二者的mmp2蛋白表达均高于肝转移灶周边正常肝组织?结论:mmp2在肝转移灶和原发灶中表达增强,有利于肿瘤细胞对基质的降解及其迁移?Mmp2基因上调使得肝转移病灶中mmp2 mrna的表达高于大肠癌原发病灶,可能是肝转移的分子机制之一?而肝转移灶周边正常肝组织中mmp2表达增强,可能与肝脏再转移有关?
英文摘要:
      objective:to explore the significance of matrix metalloproteinase 2(mmp2) gene in colorectal cancer with liver metastasis. Methods:during operation,the fresh specimens of primary cancer lesions,surrounding normal colorectal mucosas,hepatic metastases and surrounding normal liver tissues were collected in 21 patients who suffered from colorectal cancer with liver metastasis. The mrna and protein expression of mmp2 among these specimens were detected by sybr green real-time rt-pcr and western blot method. Results:there were significant differences of mrna expression level in these specimens among 21 cases,which was highest in hepatic metastases and lowest in surrounding normal colorectal mucosas. The protein expression in most of cases (18/21) were lower in surrounding normal colorectal mucosas than other specimens by western blot. There was no difference between primary cancer lesions and hepatic metastases,and both of them were higher than surrounding normal liver tissues among all cases. Conclusion:the overexpression of mmp2 gene in primary cancer lesions and hepatic metastases were beneficial for degrading the extracellular matrix. It might be one of molecular mechanisms in colorectal cancer liver metastasis that the expression of mmp2 mrna in hepatic metastases was higher than that of primary cancer lesions due to the up-regulation of mmp2 gene. The overexpression of mmp2 in normal liver tissues surrounding hepatic metastases might be correlated with the regeneration of hepatic metastasis.
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