| 胡海洋,郭宏雄,傅更锋,徐晓琴,还锡萍,羊海涛.集合HIV-1 RNA RT-PCR方法在男男性行为人群艾滋病窗口期检测中的应用[J].南京医科大学学报,2009,29(10):1395~1398 |
| 集合HIV-1 RNA RT-PCR方法在男男性行为人群艾滋病窗口期检测中的应用 |
| Application of pooled HIV-1 RNA RT-PCR in detecting HIV window period in MSM |
| 投稿时间：2009-03-31 |
| DOI：10.7655 |
| 中文关键词: 艾滋病病毒 窗口期 RT-PCR 男男性行为者 |
| 英文关键词: HIV window period pooled RT-PCR MSM |
| 基金项目:江苏省社会发展计划项目资助(BS2006078) |
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| 中文摘要: |
| 目的:检出男男性行为人群中的艾滋病病毒(HIV)窗口期感染者并初步估计发病率?方法:采用20∶1?5∶1?1∶1三级集合HIV-1 RNA RT-PCR方法进行试验?结果:检测948份男男性行为人群HIV抗体阴性血浆,发现HIV-1 RNA阳性4例,追踪随访,HIV-1抗体阳转;初步估计HIV-1年发病率约为5.55%(95%CI,3.50%~7.59%)?结论:集合HIV-1 RNA RT-PCR方法具有良好的实验效果,可以用于HIV高危人群窗口期感染者的检测? |
| 英文摘要: |
| Objective:To detect HIV-1 infection in window period and estimate HIV-1 incidence among men who have sex with men(MSM). Methods:Pooled HIV-1 RNA RT-PCR(3-staged pooling by 20∶1,5∶1,1∶1) was conducted and HIV-1 RNA copies were quantitated using COBAS AMPLICOR HIV-11.5 version MONITOR Test. Results:The plasma samples from 948 MSM,which were negative by HIV third-generation antibody assay were tested. Four samples were found to be HIV-1 RNA positive,and acute infection was diagnozed with seroconversion during follow up. The HIV-1 incidence was 5.55% per year[95%CI,3.50%~7.59%]. Conclusion:Pooled HIV-1 RNA RT-PCR is a good method of testing,and can be applied to screen HIV infection in winow-period in high-risk groups. |
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