Objective:To study the expression of TSPYL5 in gastric carcinoma and adjacent gastric tissues,and the effect of the expression of TSPYL5 on the biobehavion of gastric carcinoma. Methods:RT-PCR was used to detect the expression of TSPYL5 mRNA in 46 samples of human gastric cancer and normal gastric tissue,and the relationship between the expression of TSPYL5 and oncogenesis,lymph node metastasis,depth of tumor invasion and grade in gastric carcinoma was also analyzed. Meanwhile the effeet of TSPYL5 on AGS cells was analyzed by MTT in vivo. Results:Of 46 gastric cancer tumor tissues,26 tissues were detected the expression of TSPYL5 mRNA. The rate of TSPYL5 mRNA in the primary gastric cancer tissues was significantly higher than that in the adjacent non-cancerous tissues(56.5%). The TSPYL5 positive isolates had more lymph node metastases than that of TSPYL5 negative ones(P > 0.05). Furthermore,the expression of TSPYL5 mRNA in TNM and none metastasis signifcantly decreased(P < 0.05). TSPYL5 suppressed the growth of AGS cells. Conclusion:TSPYL5 may be had a significance effect on development and progress in gastric cancer.