文章摘要
陈〓云,刘根焰,姚〓堃,徐洁洁,卢士强,程〓冉,梁志超,崔 屹.定量检测EB病毒VCA-IgA和EA-IgA抗体对EB病毒相关鼻咽癌的诊断意义[J].南京医科大学学报,2009,29(12):1638~1642
定量检测EB病毒VCA-IgA和EA-IgA抗体对EB病毒相关鼻咽癌的诊断意义
Quantitative assay of Epstein-Barr virus specific VCA-IgA and EA-IgA antibodies for diagnosing nasopharyngeal carcinoma
投稿时间:2009-08-29  
DOI:10.7655
中文关键词: 鼻咽癌  EB病毒  病毒衣壳抗原  早期抗原  ELISA
英文关键词: nasopharyngeal carcinoma  Epstein-Barr virus  viral capsid antigen  early antigen  enzyme-linked immunoabsorbent assay
基金项目:国家自然科学基金资助(30571715,30901750);江苏省实验诊断学重点实验室基金(XK200731);江苏省人事厅六大人才高峰资助(07-B-037)
作者单位
陈〓云 南京医科大学微生物免疫学系,江苏 南京〓210029 
刘根焰 南京医科大学第一附属医院检验科,江苏 南京〓210029 
姚〓堃 南京医科大学微生物免疫学系,江苏 南京〓210029 
徐洁洁 南京医科大学第一附属医院耳鼻咽喉科,江苏 南京〓210029 
卢士强 南京医科大学微生物免疫学系,江苏 南京〓210029 
程〓冉 江苏省肿瘤医院检验科,江苏 南京〓210009 
梁志超 江苏省肿瘤医院检验科,江苏 南京〓210009 
崔 屹 中国药科大学生命科学与技术学院,江苏 南京 210009 
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中文摘要:
      目的:评价EB病毒特异性VCA-IgA和EA-IgA抗体定量检测对EB病毒相关的鼻咽癌的诊断意义?方法:试验组为228例鼻咽癌血清标本,对照组为102例正常健康体检者血清标本?用ELISA法定量检测血清标本中EBV特异性VCA-IgA和EA-IgA抗体水平,以临床病理诊断为金标准,分析各血清检测指标的准确性?灵敏性?特异性及其cut-off值的设定?结果:正常健康人血清中EB病毒特异性VCA-IgA和EA-IgA抗体的平均水平分别为15 U/ml 和 11.5 U/ml,鼻咽癌患者血清中VCA-IgA和EA-IgA抗体的平均水平分别为56.5 U/ml 和 63.5 U/ml,鼻咽癌组抗体水平显著高于健康体检组?根据VCA-IgA?EA-IgA定量检测结果绘制ROC曲线图,曲线下面积分别为0.903 和 0.948,VCA-IgA?EA-IgA定量检测可用于鼻咽癌的准确诊断?根据ROC曲线图分析,cut-off值分别设定为30 U/ml 和23 U/ml?按此值设定时,VCA-IgA检测鼻咽癌的敏感性和特异性分别为84.9%和66.8%,EA-IgA检测鼻咽癌的敏感性和特异性分别为87.9%和91.1%?联合检测VCA-IgA和EA-IgA的敏感性为92%,特异性为91%?结论:以ELISA法定量检测VCA-IgA?EA-IgA的血清抗体水平对鼻咽癌具有诊断意义,尤其将两指标联合应用?
英文摘要:
      Objective:To evaluate the diagnostic value of quantitative assay of EB virus specific antibodies to VCA-IgA and EA-IgA on diagnosing and screening of EB virus related nasopharyngeal carcinoma (NPC). Methods:The test panel consisted of 228 sera from NPC patients and 102 sera of healthy controls. For each specimen,immunoglobulin A antibodies to EBV-specific VCA(viral capsid antigen) and EA(early antigen) were quantitatively tested with enzyme linked immune absorbed assay respectively. Compared with the clinical pathologic diagnosis,the accuracy,sensitivity,specificity and cut-off value of each biomarker were analyzed. Results:The mean value of EB virus specific VCA-IgA and EA-IgA antibodies were 15 U/mL and 11.5 U/ml among healthy population;56.5 U/ml and 63.5 U/ml among NPC patients,each one’s level was significant higher in NPC patients than that in healthy population. VCA-IgA and EA-IgA can be used in diagnosing NPC accurately with 0.903 and 0.948,area under the ROC curve respectively. Through ROC curve analysis,the best cut-off values of VCA-IgA and EA-IgA were 30 U/mL and 23 U/ml. At this level,the sensitivity and specificity for diagnosing NPC were 84.9% and 66.8% for VCA-IgA,87.9% and 91.1% for EA-IgA,and 92% and 91% for combined determination. Conclusion:Quantitative detection of VCA-IgA and EA-IgA antibodies with ELISA method is very valuable in diagnosing NPC,especially combined determination.
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