文章摘要
陆明洁,洪婷婷,葛红梅,束永前,刘〓平.OPRT,DPYD,TS的mRNA水平与胃癌对氟尿嘧啶敏感性的相关性研究[J].南京医科大学学报,2009,29(12):1663~1667
OPRT,DPYD,TS的mRNA水平与胃癌对氟尿嘧啶敏感性的相关性研究
Gene expression of fluorouracil metabolic enzymes in gastric cancer correlation with ex vivo chemosensitivity towards fluorouracil
投稿时间:2009-08-19  
DOI:10.7655
中文关键词: 胃癌  氟尿嘧啶  乳清酸磷酸核糖转移酶  二氢嘧啶脱氢酶  胸腺嘧啶合成酶  ATP生物荧光法
英文关键词: stomach cancer  5-fluorouracil  orotate phosphoribosyltransferase  dihydropyrimidine dehydrogenase  thymidylate synthase  ATP-TCA
基金项目:江苏省科技厅社会发展重点项目(BS2007011)
作者单位
陆明洁 南京医科大学第一附属医院肿瘤科,江苏 南京〓210029 
洪婷婷  
葛红梅  
束永前  
刘〓平  
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中文摘要:
      目的:探讨胃癌细胞中乳清酸磷酸核糖转移酶(orotate phosphoribosyltransferase,OPRT)?二氢嘧啶脱氢酶(dihydropyrimidine dehydrogenase,DPYD)?胸腺嘧啶合成酶(thymidylate synthase,TS)的表达水平对氟尿嘧啶药物敏感性的预测价值?方法:采用ATP生物荧光法检测47例胃癌原代细胞对氟尿嘧啶的体外药物敏感性,RT-PCR法检测胃癌细胞内OPRT?DPYD?TS的mRNA表达水平,并分析胃癌细胞对氟尿嘧啶体外敏感性与这3种酶表达的关系?结果:OPRT的mRNA表达水平为0.74±0.36,DPYD的mRNA表达水平为0.85±0.35,TS的mRNA表达水平为0.57±0.28?药敏指数与OPRTmRNA表达水平呈正相关(rs=0.744,P=0.001);与TSmRNA表达水平呈负相关(rs=-0.375,P=0.009);与DPYDmRNA表达水平呈负相关(rs=-0.159,P=0.287),但无统计学显著性?结论:胃癌细胞中OPRT和TSmRNA的表达水平对氟尿嘧啶药物的治疗敏感性可能具有较好的预测价值?
英文摘要:
      Objective:To investigate the potential role of orotate phosphoribosyltransferase(OPRT),dihydropyrimidine dehydrogenase(DPYD) and thymidylate synthase(TS) in serving as biomarkers for drug sensitivity in primary gastric cancer. Methods:Viable tumor cells obtained from the samples of forty-seven patients with gastric carcinoma were tested for their sensitivity to 5-fluorouracil using ATP-TCA assay. Reverse transcription-PCR was used to measure the mRNA level of the three 5-fluorouracil related genes. Results:OPRT mRNA level was positively correlated with the sensitivity to 5-fluorouracil (r=0.744,P=0.001) while TS mRNA level was negatively correlated with the sensitivity(r=-0.375,P=0.009). No significant association was observed between DPYD expression and 5-fluorouracil sensitivity (r=-0.159,P=0.287). Conclusion:Detection of the expression level of OPRT and TS mRNA in gastric cancer might be of good value for predicting the sensitivity to 5-fluorouracil.
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