文章摘要
蔡红星,朱正秋,朱祖安.尼美舒利对人胃癌SGC-7901细胞凋亡影响的实验研究[J].南京医科大学学报,2009,29(12):1668~
尼美舒利对人胃癌SGC-7901细胞凋亡影响的实验研究
The study on the mechanism of nimesulide on apoptosis of human gastric carcinoma SGC-7901 cells
投稿时间:2009-08-19  
DOI:10.7655
中文关键词: 环氧合酶2  胃癌  非甾体抗炎药  尼美舒利  bcl-2  bax
英文关键词: COX-2  gastric carcinoma  NSAIDs  nimesulide  bcl-2  bax
基金项目:江苏省高校自然科学基金资助(05KJD320231)
作者单位
蔡红星 徐州医学院法医学教研室,江苏 徐州〓221004 
朱正秋 徐州医学院附属医院肿瘤内科,江苏 徐州〓221002 
朱祖安 徐州医学院附属医院消化内科,江苏 徐州〓221002 
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中文摘要:
      目的:观察尼美舒利对人胃癌SGC-7901细胞凋亡的影响并对其机制进行初步研究?方法:体外培养人胃癌SGC-7901细胞,并给予不同浓度尼美舒利干预,HE染色?电镜观察药物作用后细胞形态学变化;免疫组化?Western blot方法检测药物对人胃癌SGC-7901细胞COX-2?bcl-2?bax蛋白表达的影响?结果:HE染色检测结果显示随着给药浓度的上升及给药时间的延长,SGC-7901细胞逐渐变得稀疏,光镜及电镜下高浓度组可见核固缩?核碎裂?染色质边集等凋亡及坏死的形态学表现;免疫组化?Western blot结果显示尼美舒利作用48 h后人胃癌SGC-7901细胞COX-2?bcl-2表达逐渐下降,bax表达逐渐上升,具有剂量依赖性?COX-2与bcl-2之间蛋白表达率具有相关性(rs = 0.885 9;P < 0.001)?结论:尼美舒利可以促进人胃癌SGC-7901细胞凋亡,其促凋亡机制与促进bax表达及抑制bcl-2表达有关?
英文摘要:
      Objective:To investigate the mechanism of nimesulide on human gastric carcinoma SGC-7901 cells apoptosis. Methods:SGC-7901 cells were incubated in RPMI 1640 containing different concentration of nimesulide(0,12.5,50.0,100.0,200.0,400.0 μmol/L). After treated with nimesulide,the cytologic morphological changes were observed by the optical microscope and transmission electron microscopy. The Power VisionTM two step method was used for the immunostaining of COX-2,bcl-2,bax. Westernblot analysis was employed to investigate the expression of COX-2,bcl-2,bax. Results:Through HE staining,the cells in negative control groups had normal structures,but with the increase of dose of nimesulide,the SGC-7901cells became sparse. In high concentration group,the dead cells showed morphologic changes of apoptosis,such as nuclear condenstion,fragmentation and chromatin crescent. By electron microscopy,cells in high concentration group also had apoptotic characteristics including chromatin condensation,nucleus fragmentation,chromatin crescent and apoptosis body. With the increase of concentration of nimesulide,the expressions of COX-2,bcl-2 decreased,while the level of bax protein increased by immunohistochemical and Western blot assay. There was positive correlation between the expression of COX-2 and bcl-2(rs=0.885 9;P < 0.001). Conclusion:Nimesulide can induces apoptosis,which may be related to overexpression of bax and inhibition of bcl-2.
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