Objective:To examine CFA(complete Freund’s adjuvant)-induced IP-10 expression in the paw skin and the regulation of c-jun N-terminal kinase(JNK) inhibitor on CFA-induced pain and IP-10 expression. Methods:CFA was injected into the rat’s unilateral hind paw to induce chronic inflammatory pain. Heat hyperalgesia on both hind paws were tested at different time points after injection of CFA. Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to examine the expression of IP-10 mRNA and protein in the paw skin respectively. By intraplantar injection,the effect of JNK inhibitor SP600125 on CFA-induced pain and IP-10 upregulation was detected. Results:Peripheral injection of CFA induced persistent heat hyperalgesia. Both IP-10 mRNA and protein expression were increased in the paw skin at the time points of 3 h,6 h and 1 d after CFA injection. Intraplantar injection of SP600125 attenuated CFA-induced heat hyperalgesia and reduced IP-10 expression. Conclusion:IP-10 is involved in CFA-induced pain. JNK plays a role in pain and IP-10 expression induced by CFA.