文章摘要
赵〓莹,王海昌,张荣庆.不同浓度葡萄糖环境下Fe2+对人脐静脉平滑肌细胞增殖及基质金属蛋白酶-2分泌的促进作用[J].南京医科大学学报,2010,(2):170~173
不同浓度葡萄糖环境下Fe2+对人脐静脉平滑肌细胞增殖及基质金属蛋白酶-2分泌的促进作用
Promotion of ferrous ion on the secretion of matrix metalloproteinases-2 and the proliferation of HUVSMC in different glucose concentration milieu
  
DOI:10.7655
中文关键词: 亚铁离子  人脐静脉平滑肌细胞  葡萄糖  支架内再狭窄  基质金属蛋白酶-2
英文关键词: Fe2+  HUVSMC  glucose  in-stent restenosis  MMP-2
基金项目:国家自然科学基金资助(30770784)
作者单位
赵〓莹 武汉市普爱医院心内科,湖北 武汉〓430034 
王海昌 第四军医大学西京医院心内科,陕西 西安〓710032 
张荣庆 第四军医大学西京医院心内科,陕西 西安〓710032 
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中文摘要:
      目的:探讨正常及高葡萄糖环境下,Fe2+对人脐静脉平滑肌细胞(HUVSMC)增殖及基质金属蛋白酶-2(MMP-2)分泌的作用?方法:以相同浓度接种普通培养液及高浓度葡萄糖培养液培养的3~5代HUVSMC为正常组及高糖组,分别以不同浓度Fe2+处理?培养24 h后,四甲基氮唑盐(MMT)比色法测定细胞增殖水平,ELISA试剂盒检测细胞培养上清中MMP-2含量?结果:不同浓度葡萄糖环境下,0.012 5~0.100 0 g/L浓度Fe2+均能促进HUVSMC增殖,且高糖组作用较正常组强;正常组Fe2+浓度在0.1 g/L时能促进HUVSMC分泌MMP-2,高糖组所有浓度Fe2+均表现促分泌作用?结论:适当浓度的Fe2+能影响HUVSMC的生物学特性,促进HUVSMC的增殖与MMP-2的分泌,且高糖环境下促进作用更为显著?
英文摘要:
      Objective:To investigate the effects of Fe2+on the proliferation of the HUVSMC and its secretion of MMP-2 under the condition of normal or high glucose. Methods:Primarily cultured HUVSMC(Passage 3~5)were cultured with the normal and high glucose DEME,and then subjected to control or Fe2+ incubation at various concentrations(0.006 25 g/L,0.012 5 g/L,0.025 g/L,0.05 g/L,0.1 g/L). After 24 h of intervention,HUVSMC proliferation was assessed with MTT method,and MMP-2 secretion was measured by ELISA. Results:Compared to the control group:in normal glucose group,HUVSMC proliferation in 0.012 5~0.1 g/L Fe2+ treatment group was increased(P < 0.01),and the secretion of MMP-2 was promoted only in 0.1 g/L Fe2+ treatment group(P < 0.01);in high glucose group:HUVSMC proliferation in 0.012 5~0.1 g/L Fe2+ treatment group was increased(P < 0.05),and the secretion of MMP-2 was promoted in all experimental groups(P < 0.05);and the proliferation of HUVSMC and level of MMP-2 in high glucose group were higher than those in the normal glucose group(P < 0.05). Conclusion:Fe2+ could stimulate the proliferation of VSMC,as well as the secretion of MMP-2. And high glucose may contribute to that accelerated effects.
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