文章摘要
成志勇,牛志云,李〓琳,魏占惠,魏玉涛,董作仁,潘〓崚.PTEN?VEGF?FLT1基因在髓系白血病中的表达及其相关性研究[J].南京医科大学学报,2010,(2):234~
PTEN?VEGF?FLT1基因在髓系白血病中的表达及其相关性研究
The correlation and significance of mRNA expression of PTEN,VEGF,FLT1 in myeloid leukemia
  
DOI:10.7655
中文关键词: PTEN基因  血管内皮细胞生长因子  血管内皮细胞生长因子受体1  髓系白血病
英文关键词: PTEN gene  VEGF  FLT1  myeloid leukemia
基金项目:河北省自然基金资助项目(C200500745)
作者单位
成志勇 保定市第一医院血液内科,河北 保定〓071000 
牛志云 河北医科大学第二医院血液内科,河北 石家庄〓050000 
李〓琳 保定市第一医院血液内科,河北 保定〓071000 
魏占惠 保定市第一医院血液内科,河北 保定〓071000 
魏玉涛 保定市第一医院血液内科,河北 保定〓071000 
董作仁 河北医科大学第二医院血液内科,河北 石家庄〓050000 
潘〓崚 河北医科大学第二医院血液内科,河北 石家庄〓050000 
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中文摘要:
      目的:探讨肿瘤抑制基因PTEN及血管内皮生长因子(VEGF)及其受体FLT1(VEGFR1)在白血病中的表达及其相关性?方法:应用实时荧光定量PCR(FQ-PCR)检测50例初治急性髓系白血病(AML),10例缓解期AML,10例慢性粒细胞白血病慢性期(CML-CP)?10例CML急变期(CML-BC)患者和10例正常人骨髓单个核细胞(MMNCs)中PTEN?VEGF?FLT1 mRNA的表达?结果:初治AML患者PTEN mRNA表达水平明显低于缓解组AML和正常对照组,CML-BC患者PTEN mRNA表达水平低于CML-CP患者,在AML初治白血病中PTEN mRNA与VEGF和FLT1 mRNA表达负相关,PTEN低表达?VEGF和FLT1高表达与AML缓解率及髓外浸润相关?结论:髓系白血病患者中低表达PTEN基因,高表达VEGF及FLT1基因,并与白血病预后不良及髓外浸润密切相关,三者相互作用共同参与了白血病的发病?
英文摘要:
      Objective:To investigate the clinical significance and relationship of tumor-suppressing gene PTEN and VEGF,FLT1(VEGFR1) mRNA in myleod leukemia cells. Methods:The mRNA expression of PTEN and VEGF in marrow mononuclear cells (MMSCs) from 50 acute myeloid leukemia (AML) patients,10 AML patients in complete remission (CR),10 chronic myelogenous leukemia (CML) patients in chronic phase (CML-CP),10 CML patients in blast crises (CML-BC) and 10 persons as normal controls were detected by real-time fluorescent relative-quantification PCR(FQ-PCR). The correlation and clinical significance of PTEN,VEGF and FLT1 were evaluated. Results:The expression level of PTEN mRNA was lower in AML patients than normal control;and it was lower in CML-BC patients than that in CML-CP patients. PTEN mRNA expression level was negatively correlated with VEGF and FLT1 mRNA,and the low-expression of PTEN gene and high-expression of VEGF and FLT1 gene were associated with the poor prognosis and the higher extra-medullary infiltrations. Conclusion:The low-expression of PTEN mRNA and high-expression of VEGF or FLT1 mRNA in myleod leukemia meand the poor prognosis and the higher extra-medullary infiltrations. PTEN,VEGF and FLT1 mRNA may have synergistic effect on the pathogenesis of myleod leukemia.
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