文章摘要
刘敏星,张〓铮,秦海东,徐〓英,宋〓希,范晓春.血必净对肺血栓栓塞大鼠P-选择素表达的影响[J].南京医科大学学报,2010,(3):371~374415
血必净对肺血栓栓塞大鼠P-选择素表达的影响
Effects of Xuebijing injection on the expression of P-selectin of rat with pulmonary thromboembolism
投稿时间:2009-10-15  
DOI:10.7655
中文关键词: 肺血栓栓塞  P-选择素  血必净注射液
英文关键词: pulmonary thromboembolism  P-selectin  Xuebijing injection
基金项目:南京市医学科技发展重大项目
作者单位
刘敏星 南京医科大学附属南京第一医院急诊科,江苏 南京〓210006 
张〓铮  
秦海东  
徐〓英  
宋〓希  
范晓春  
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中文摘要:
      目的:观察大鼠肺血栓栓塞症中血清?肺血管内皮细胞上P-选择素(P-selectin,Ps)的变化,并探讨血必净注射液对其的影响?方法:颈静脉注入自体血凝块制备动物模型?将105只健康雄性SD大鼠按随机数字表法分为对照组?栓塞组和血必净组?每组又分为术后1 h?3 h?1天?3天?7天 5个时间段,每个时间段7只?留取肺组织和血清,观察各组肺组织病理变化,Western blot法检测肺血管内皮细胞上Ps的蛋白表达水平,ELISA法测血清中可溶性P-选择素(sP-selectin,sPs)含量?结果:肺栓塞后病理可见肺动脉中血栓栓塞,炎症反应明显?肺血管内皮上Ps的蛋白表达水平及血清中sPs含量在栓塞后1 h开始显著增高(P < 0.01)?血必净组经治疗后,病理损伤减轻,肺血管内皮上Ps的蛋白表达水平于1 h后下降,与栓塞组比较,有显著性差异(P < 0.01),血清sPs含量也下降,与栓塞组比较,1天后差异均有统计学意义(P < 0.01或P < 0.05)?结论:血必净注射液可能通过降低肺血栓栓塞时Ps表达,从而减轻炎症和凝血纤溶失衡对肺组织的损害,对肺组织具有一定的保护作用?
英文摘要:
      Objective:To observe and discuss the effects of Xuebijing injection on the expression of P-selectin in serum and on the pulmonary vascular endothelial cells of PTE model rats. Methods:The PTE model was established by intravenous injection of autologous blood clots. One hundred and five healthy male SD rats were randomly divided into control group,PTE model group,and Xuebijing-treated group. Every group were divided into 5 subgroups according to the time of 1 hour,3 hour,1 day,3 day and 7 day after opreation,with 7 rats in each subgroup. Pathological changes of lung tissue were observed,and the expression of P-selectin protein on the pulmonary vascular endothelial cells was evaluated by Western blot. The contents of sP-selectin in serum were detected by ELISA. Results:The notable embolism in pulmonary arteries and inflammatory cell infiltration around alveoli could be found by microscopy. The expression of P-selectin protein on the pulmonary vascular endothelial cell and the contents of P-selectin in serum significantly elevated from 1 hour after embolization (P < 0.01). Whereas after using Xuebijing injection,the pathological injury was ameliorated.The expression of Ps protein on the pulmonary vascular endothelial cells were significantly decreased from 1 hour after embolization (P < 0.01). The contents of P-selectin in serum were also decreased and the differences between PTE model group and Xuebijing-treated group were statistically significant from 1 day after embolization(P < 0.01 or P < 0.05). Conclusion:Xuebijing injection may reduce the damage of inflammation and coagulation-fibrinolysis imbalance to the lung tissue through decreasing the expression of P-selectin in PTE,so it has a certain protective effect to the lung tissue.
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