Objective:To study the gene mutation of thyroid hormone receptorβ(TRβ)in a family with thyroid hormone resistance syndrome accompanied by Hashimoto’s thyroiditis. Methods:The peripheral blood samples of the patient,9 family members and 9 healthy subjects were collected,then thyroid function was detected and the genomic DNA was isolated. The exons 1~10 of TRβ gene were amplified by PCR. The products of PCR were purified and sequenced directly to detect the gene mutation. Results:No mutation was found in exon 1~10 of TRβ gene. Conclusion:The mutation of TRβ gene is not only reason for the pathogenesis of thyroid hormone resistance syndrome.