文章摘要
杨留才,李玉华,李仕红,林〓辉.性激素浓度?雌激素受体及其β基因多态性与青年人胃腺癌发生发展的相关性研究[J].南京医科大学学报,2010,(3):399~404
性激素浓度?雌激素受体及其β基因多态性与青年人胃腺癌发生发展的相关性研究
Correlation of sex hormones concentration,ER receptor and the polymorphism of ERβ gene with development of gastric adenocarcinoma in young patients
  
DOI:10.7655
中文关键词: 青年胃腺癌  发展  性激素  血清  雌激素受体β基因多态性
英文关键词: gastric adenocarcinoma  development  sex hormone  the polymorphism of ERβ gene
基金项目:2008年盐城市医学科技发展计划项目资助(YK2008121)
作者单位
杨留才 盐城卫生职业技术学院生物化学与分子生物学系,江苏 盐城〓224006 
李玉华 盐城卫生职业技术学院生物化学与分子生物学系,江苏 盐城〓224006 
李仕红 盐城卫生职业技术学院生物化学与分子生物学系,江苏 盐城〓224006 
林〓辉 盐城市第一人民医院核医学科,江苏 盐城〓224000 
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中文摘要:
      目的:探讨性激素浓度及其受体和雌激素受体β(ERβ)基因多态性与青年人胃腺癌发生发展的关系?方法:以原发性青年胃腺癌患者男女各108例为实验组,随机抽取住院手术胃溃疡患者男女各80例为对照组,用放射免疫法(RIA)检测两组血清性激素水平;用免疫组织化学法(SP法)检测两组雌激素受体(estrogen receptor,ER)表达状况,并用聚合酶链反应-限制性片断长度多态性技术(PCR-RFLP)检测ERβ基因多态性?结果:实验组血清雄激素高于对照组,但雌激素低于对照组,差异均有统计学意义(P < 0.000 1)?实验组男女性患者,在TNM分期之间差异无统计学意义(P > 0.05),但雄激素递减,雌激素递增;而随分化程度降低?淋巴结转移阴性和阳性之间,差异均有统计学意义(P < 0.01)?对照组ERα和ERβ均不表达,而实验组ERα和ERβ阳性率分别为57.4%(124/216)和15.7% (34/216),经统计学处理具有显著性差异(P < 0.000 1),其中分化程度低?有淋巴结转移者?浸润程度深的阳性率高于分化程度高?无淋巴结转移者?浸润程度浅的,且TNM Ⅲ期高于Ⅰ?Ⅱ期(P < 0.001)?PCR-RFLP检测中,用Rsa I酶切显示实验组RR和Rr基因型显著高于rr型,并以RR基因型最高(P < 0.000 1),且分化程度低?有淋巴结转移和浸润越深者RR高,TNM分期Ⅲ期高于Ⅰ?Ⅱ期(P < 0.000 1);Alu I酶切显示各基因型差异无统计学意义 (P > 0.05)?结论:血清性激素水平改变是影响胃腺癌变和易感性的重要因素之一,ERβ基因多态性可能与胃癌的发生发展密切相关?
英文摘要:
      Objective:To explore the correlation of sex hormones concentration,ER receptor,and the polymorphism of ERβ gene with the development of gastric adenocarcinoma in young people. Methods:Two hundred and sixteen cases of primarily gastric adenocarcinoma in young people and 160 cases of gastrohelcoma surgery patients were analyzed. Radioimmunoassay(RIA) was used to determine the levels of sex hormone. Immunohistochemistry method was used to detect the expression of ER. Polymerase chain reaction-restriction fragment length polymorphism method was used to detect the polymorphism of ERβ of test group. Results:Serum androgenic hormone levels were significantly lower in the control group than that of the test group,but serum estradiol levels were higher in the control group than that of the test group. No significant difference can be seen in the TNM staging in the test group between male and female. But androgenic hormone levels decreased progressively,estradiol levels increased progressively with differentiation degree degraded,and there are significant difference between positive and negative lymph node metastasis. There was negative expression of ERα and ERβ in control group. The positive rate of ERα and ERβ in the test groups were 57.4%(124/216) and 15.7%(34/216). The positive rates of ER were significantly higher in the tissue with lower differentiation degree,having lymph node metastasis and deeper infiltration degree than that with higher differentiation degree,having no lymph node metastasis and shallower infiltration degree. Moreover,the positive rates of ER were higher in the stage of TNM Ⅲ than in the stage of TNMⅠandⅡ. RR and Rr genetype rate were higher than rr genetype rate. RR genetype was most obviously observed by PCR-RFLP with the allelomorphic gene distribution of RsaI. RR were significantly higher in the tissue with lower differentiation degree,having lymph node metastasis,deeper infiltration degree,and with the stage of TNM Ⅲ than that with higher differentiation degree,having no lymph node metastasis,shallower infiltration degree,and with stages ofⅠandⅡ. There was no significant difference in all genetypes with the allelomorphic gene distribution of Alu I in the test group. Conclusion:The alteration of sex hormones concentration in serum is one of important factors effecting the cancerization of gastric gland. The polymorphism of ERβ gene may be related to the development of gastric carcinoma.
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