文章摘要
周晓杰,苗登顺.活性维生素D缺乏促进小鼠皮肤衰老[J].南京医科大学学报,2010,(5):591~596
活性维生素D缺乏促进小鼠皮肤衰老
Active vitamin D deficiency resulting in skin aging
投稿时间:2009-12-09  
DOI:10.7655
中文关键词: 1,25(OH)2D3  1α羟化酶  基因敲除  皮肤衰老
英文关键词: 1,25(OH)2D3  1α-hydroxylase  gene knock-out  skin aging
基金项目:江苏省卫生厅科研项目(H200627)
作者单位
周晓杰 南京医科大学人体解剖学系,江苏 南京 210029 
苗登顺  
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中文摘要:
      目的:探讨1,25二羟基维生素D3[1,25(OH)2D3]在抗皮肤衰老中的作用及机制?方法:使用组织学?免疫组织化学?免疫印迹和生化指标检测的方法比较同窝10周龄25-羟化维生素D 1α-羟化酶基因敲除纯合子[1α(OH)ase-/-]小鼠和野生型小鼠皮肤表型的差异?结果:1α(OH)ase-/-小鼠皮肤衰老相关β-半乳糖苷酶染色增强,出现皮肤变薄?表真皮交界平坦?皮下脂肪和毛囊数目减少等衰老表型;通过细胞增殖性核抗原(PCNA)免疫组织化学染色和TUNEL检测,发现1α(OH)ase-/-小鼠皮肤中PCNA阳性细胞明显减少而TUNEL阳性细胞则显著增多;免疫印迹结果显示p16?p27?p53?NF-?资B?活化型Caspase-3在1α(OH)ase-/-小鼠皮肤组织中表达明显升高;生化检测显示1α(OH)ase-/-小鼠皮肤活性氧(ROS)水平明显升高?超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)水平降低?丙二醛(MDA)含量增高?结论:1,25(OH)2D3能够通过促进皮肤细胞增殖?抑制皮肤细胞凋亡和抗氧化应激作用发挥抗皮肤衰老的作用?
英文摘要:
      Objective:To investigate the mechanism of 1,25(OH)2D3 in preventing skin aging. Methods:The phenotype of skin was compared between wild-type mice and 1α-hydroxylase gene knock-out littermates at 10 weeks of ages by histology,immunohistochemistry,western-blot and biochemical analysises,respectively. Results:1α-hydroxylase gene knock-out mice displayed a premature aging phenotype in skin including thinner skin with increased senescence associated β-galactosidase activity,flattening of the dermal-epidermal junction,reduced subcutaneous fat deposition and hair follicles number. Proliferating cell nuclear antigen(PCNA) immunopositive cells in skin were decreased, but terminal deoxynucleotidyl transferase dUTP nick end labeling positive areas were increased significantly in 1α-hydroxylase gene knock-out mice compared to their wild-type littermates. The expression of p16,p27,p53,NF-?资B,active caspase-3 was up-regulated markedly in skin from 1α-hydroxylase gene knock-out mice. Reactive oxygen species and malondialdehyde levels were raised,whereas activities of superoxide dismutase and glutathione peroxidase were reduced in 1α-hydroxylase gene knock-out mice. Conclusion:1,25(OH)2D3 plays an important role in preventing skin aging by stimulating cell proliferation,inhibiting cells apoptosis and reducing oxidative stress.
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