文章摘要
任戈亮,桂鉴超,王黎明,徐 燕,蒋逸秋,高航飞.Ⅱ型胶原羧基端端肽对骨关节炎早期诊断和病情评估的实验研究[J].南京医科大学学报,2010,(5):653~657
Ⅱ型胶原羧基端端肽对骨关节炎早期诊断和病情评估的实验研究
An experimental study of C telopeptide of type Ⅱ collagen for early diagnosis and condition evaluation of osteoarthritis
  
DOI:10.7655
中文关键词: 骨关节炎  软骨  Ⅱ型胶原  CTX-Ⅱ
英文关键词: osteoarthritis  cartilage  collagen typeⅡ  CTX-Ⅱ
基金项目:南京医科大学科技发展基金重点资助项目(06NMUZ037)
作者单位
任戈亮 南京医科大学附属南京第一医院骨科,江苏 南京 210006 
桂鉴超  
王黎明  
徐 燕  
蒋逸秋  
高航飞  
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中文摘要:
      目的:定量检测骨关节炎(OA)大鼠模型血清?关节软骨蛋白提取液中Ⅱ型胶原羧基端端肽(CTX-Ⅱ)的变化,探讨其作为OA早期诊断及病情评估指标的价值和意义?方法:SD大鼠行前交叉韧带切断术诱导OA模型,将大鼠随机分为模型组和假手术组?于造模后不同时间点分批取血并处死取关节标本,检测血清及软骨蛋白提取液中CTX-Ⅱ水平,观察病理变化?结果:模型组术后2周开始出现OA特征性病理变化且逐渐加重,CTX-Ⅱ水平在术后3天开始不断升高?两组血清及软骨蛋白提取液中CTX-Ⅱ水平在造模后各时间点均有统计学差异,血清和软骨蛋白提取液中CTX-Ⅱ水平变化具有相关性?结论:在大鼠OA模型中,血清CTX-Ⅱ水平在未出现明显病理变化时即已开始增高,并与病灶区软骨中CTX-Ⅱ表达一致,可作为OA早期诊断和病情评估的参考指标?
英文摘要:
      Objective:To observe the expression of C telopeptide of type Ⅱcollagen(CTX-Ⅱ)in serum and protein extract from joints of experimental osteoarthritis(OA),and to explore the value of CTX-Ⅱ as a biomarker in early diagnosis and condition assessment. Methods:96 adult SD rats were randomly divided into two groups:Sham-operated group (n=48),OA model group(n=48). OA was induced by unilateral surgical transection of the anterior cruciate ligament. Joints were isolated 0,3days,1,2,4,8weeks after surgery for protein extraction and histology. CTX-Ⅱ in protein extracts and serum was measured by ELISA. Results:Levels of CTX-Ⅱ in serum and protein extract were both significantly increased in OA model group compared with sham-operated group on each time point. The pathological features of OA were observed during 2~8 weeks and became gradually more serious. The changes in level of CTX-Ⅱ in serum and protein extraction showed a close correlation. Conclusion:Serum CTX-Ⅱ level increases before the appearance of characteristic pathology of OA and is in coincidence with the changes of CTX-Ⅱ in the tissue. It can be a sensitive reference indicator for early diagnosis and condition evaluation of OA.
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