文章摘要
陈 瑜,刘东方,叶 果.Graves病和桥本甲状腺炎患者甲状腺原位凋亡和Fas/FasL的表达[J].南京医科大学学报,2010,(5):673~677
Graves病和桥本甲状腺炎患者甲状腺原位凋亡和Fas/FasL的表达
The determination of apoptosis and expression of Fas/FasL in thyroid cells of AITD
  
DOI:10.7655
中文关键词: Graves病  桥本甲状腺炎  甲状腺  凋亡  凋亡蛋白
英文关键词: Graves disease  hashimoto thyroiditis  thyroid gland  apoptosis  apoptosis protein
基金项目:重庆市自然科学基金(CSTC2006BB5293)
作者单位
陈 瑜 重庆市肿瘤研究所内科,重庆 400030 
刘东方 重庆医科大学附属第二医院内分泌科,重庆 400010 
叶 果 重庆市肿瘤研究所内科,重庆 400030 
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中文摘要:
      目的:探讨正常人?Graves病(GD)和桥本甲状腺炎(HT)患者甲状腺细胞(TEC)凋亡率的差异,并观察凋亡相关蛋白的表达情况?方法:TUNEL法检测3种甲状腺组织的TEC凋亡率;免疫组化检测TEC凋亡蛋白Fas?FasL表达情况,RT-PCR检测Fas mRNA表达的差异?结果:GD和HT患者TEC凋亡率显著高于正常对照(P < 0.05),HT患者TEC凋亡率较GD患者也显著升高(P < 0.05),FasL在正常?GD和HT甲状腺组织中存在连续性表达,但Fas 在自身免疫性甲状腺疾病(AITD)患者的表达水平显著高于正常甲状腺组织,以HT的Fas表达水平最高(HT与正常对照相比,P < 0.01;GD与正常对照比,P < 0.05)?结论:凋亡可能在AITD的发病中起一定作用,Fas及其配基系统是与AITD TEC凋亡相关的蛋白?
英文摘要:
      Objective:To explore the apoptosis rate of thyroid cells from normal,Graves disease(GD),Hashimoto thyroiditis(HT) and detect the apoptosis related proteins. Methods:The apoptosis rate of thyroid cell from normal GD and HT thyroid gland was measured by the terminal deoxynucleotidyl transferase mediated d-UTP nick labeling(TUNEL),and apoptosis related proteins Fas and FasL were confirmed by immunohistochemical methods,and the expression of Fas mRNA was measured by RT-PCR. Results:Higher apoptosis rate was found in GD and HT TEC,and there was an obvious and almost equal expression of FasL(P > 0.05). The difference in expression of Fas protein and mRNA in three groups thyroid glands was significant (P < 0.01),and the expression of Fas in normal tissue was the lowest and trivial,but the Fas expression in HT was the highest and distinctive. Conclusion:These data indicate that apoptosis plays an important role in the pathogenesis of AITD,and Fas/FasL may be the signal molecule related to apoptosis of TEC.
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