Objective:To investigate the expression and significance of microRNA 200c (miR-200) and microRNA 141 (miR-141) in bladder transitional cell cancer. Methods:MicroRNA array was used to screen microRNA differentiated expressed between bladder cancer and normal peri-cancer tissue. The expression of miR-200c and miR-141 in bladder cancer samples and bladder cancer cells was determined using real-time quantitative reverse transcription (qRT)-PCR. Results:MiR-200c and miR-141 quantification showed higher expression of miR-200c and miR-141 in bladder cancer samples than normal peri-cancer tissue. The expression of miR-200c and miR-141 was higher in superficial baldder cancer than invasive bladder cancer;it was also higher in bladder cancer sample with higher grade than lower grade. Likewise,5637 cell,which is relative well-differentiated and less malignant,showed higher expression of miR-141 and miR-200c than T24,J82 and EJ cell,which were relative poor-differentiated and more malignant. Conclusion:MiR-200c and miR-141 may be involved in the progression of bladder cancer.