文章摘要
李鹏超,陶〓俊,吕〓强,吴德尧,秦伟杰,张〓炜.MiR-200c和miR-141在膀胱移行细胞癌中的表达研究[J].南京医科大学学报,2010,(6):768~771786
MiR-200c和miR-141在膀胱移行细胞癌中的表达研究
Expression of miR-200c and miR-141 in bladder tansitional cell cancer
投稿时间:2010-02-10  
DOI:10.7655
中文关键词: miR-200  miR-141  膀胱癌
英文关键词: miR-200c  miR-141  bladder cancer
基金项目:
作者单位
李鹏超 南京医科大学第一附属医院泌尿外科,江苏 南京〓210029 
陶〓俊  
吕〓强  
吴德尧  
秦伟杰  
张〓炜  
摘要点击次数: 1785
全文下载次数: 231
中文摘要:
      目的:探讨microRNA 200c(miR-200c)和microRNA 141(miR-141)在膀胱移行细胞癌中的表达及意义?方法:MicroRNA芯片筛选膀胱癌和癌旁组织差异表达的microRNA,实时定量 RT-PCR法验证miR-200c和miR-141在膀胱癌组织和膀胱癌细胞系中的表达?结果:MiR-200c和miR-141的表达强度在膀胱肿瘤组织高于癌旁正常膀胱黏膜,在表浅性膀胱肿瘤高于肌层浸润性膀胱肿瘤,在高级别组膀胱肿瘤高于低级别组,在分化较好?恶性度较低的5637细胞系中表达较高,在分化较差?恶性度较高的T24?J82?EJ细胞系中表达均较低?结论:MiR-200c和miR-141可能参与膀胱癌的进展?
英文摘要:
      Objective:To investigate the expression and significance of microRNA 200c (miR-200) and microRNA 141 (miR-141) in bladder transitional cell cancer. Methods:MicroRNA array was used to screen microRNA differentiated expressed between bladder cancer and normal peri-cancer tissue. The expression of miR-200c and miR-141 in bladder cancer samples and bladder cancer cells was determined using real-time quantitative reverse transcription (qRT)-PCR. Results:MiR-200c and miR-141 quantification showed higher expression of miR-200c and miR-141 in bladder cancer samples than normal peri-cancer tissue. The expression of miR-200c and miR-141 was higher in superficial baldder cancer than invasive bladder cancer;it was also higher in bladder cancer sample with higher grade than lower grade. Likewise,5637 cell,which is relative well-differentiated and less malignant,showed higher expression of miR-141 and miR-200c than T24,J82 and EJ cell,which were relative poor-differentiated and more malignant. Conclusion:MiR-200c and miR-141 may be involved in the progression of bladder cancer.
查看全文   查看/发表评论  下载PDF阅读器
关闭