文章摘要
俞〓欣,刘〓敏,柏金明,徐东亮,鲁〓佩,吕〓强,张〓炜,顾〓民.大鼠慢性移植物肾病模型中GSK3β的表达及其病理意义[J].南京医科大学学报,2010,(6):772~775
大鼠慢性移植物肾病模型中GSK3β的表达及其病理意义
The expression and pathological significance of GSK3β in chronic allograft nephropathy model of rats
投稿时间:2010-03-22  
DOI:10.7655
中文关键词: 肾移植  慢性移植物肾病  GSK3β  NF-κB
英文关键词: kidney transplantation  CAN  GSK3β  NF-κB
基金项目:
作者单位
俞〓欣 南京医科大学第一附属医院泌尿外科肾移植中心,江苏 南京〓210029 
刘〓敏  
柏金明  
徐东亮  
鲁〓佩  
吕〓强  
张〓炜  
顾〓民  
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中文摘要:
      目的:观察糖原合成酶激酶-3β(glycogen synthase kinase,GSK3β)在大鼠慢性移植物肾病(chronic allograft nephropathy,CAN)模型移植肾中的表达?探讨GSK3β在CAN中的作用?方法:F344大鼠为供体,Lewis大鼠为受体,行原位异体肾移植,建立大鼠CAN模型,术后12周处死各组动物,取移植肾标本观察组织形态学变化,并用SP免疫组织化学方法对移植肾组织GSK3β?NF-κB p65的表达进行观察?结果:与同系移植组?对照组相比,CAN组GSK3β?NF-κB p65在移植肾中的表达明显增强?结论:提示GSK3β可能在CAN的发病机制中发挥重要作用?
英文摘要:
      Objective:To study the expression of GSK3β in chronic allograft nephropathy(CAN)model of rats and investigate the possible role of GSK3β in CAN. Methods:Fisher(F344) kidneys were orthotopically transplanted into Lewis rat recipients to set up chronic allograft nephropathy model in rats. Animals were sacrificed 12 weeks post-transplantation,and the grafts were harvested for histological study,and the expression of GSK3β and NF-κB p65 in renal tissue was analyzed by immunochemistry of SP method. Results:The expression levels of GSK3β and NF-κB p65 in CAN group were significantly higher than those in isograft group and control group. Conclusion:Our research suggests that GSK3β might play an important role in pathogenesis of CAN.
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