文章摘要
徐克群,薛乐宁,龚燕芳,满晓华,吴洪玉,金〓晶.急性胰腺炎大鼠抵抗素?CRP?IL-1β和TNF-α水平的变化[J].南京医科大学学报,2010,(6):827~831
急性胰腺炎大鼠抵抗素?CRP?IL-1β和TNF-α水平的变化
The variety of resistin,CRP,TNF-α and IL-1β in the rat of acute pancreatitis
投稿时间:2010-01-03  
DOI:10.7655
中文关键词: 急性胰腺炎  抵抗素  C反应蛋白  肿瘤坏死因子α  白介素1-β
英文关键词: acute pancreatitis  resistin  CRP  TNF-α  IL-1β
基金项目:常州市社会发展基金资助(CS2008212)
作者单位
徐克群 南京医科大学附属常州市第二人民医院消化科,江苏 常州〓213003 
薛乐宁 南京医科大学附属常州市第二人民医院消化科,江苏 常州〓213003 
龚燕芳 上海第二军医大学附属长海医院消化科,上海〓200433 
满晓华 上海第二军医大学附属长海医院消化科,上海〓200433 
吴洪玉 上海第二军医大学附属长海医院消化科,上海〓200433 
金〓晶 上海第二军医大学附属长海医院消化科,上海〓200433 
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中文摘要:
      目的:探讨在急性胰腺炎(AP)大鼠血清中抵抗素?C反应蛋白(CRP)?白介素1-β(IL-1β)?肿瘤坏死因子α(TNF-α)水平的变化?方法:用ELISA测定大鼠血清中抵抗素? TNF-α? IL-1β? CRP水平变化,同时检测胰腺/体重比值?胰腺组织病理变化?结果:急性水肿型胰腺炎(AEP)组和急性坏死型胰腺炎(ANP)组大鼠血清淀粉酶(AMY)?抵抗素?TNF-α?IL-1β?CRP水平较正常对照组?假手术组均明显升高,ANP组与AEP组相比升高更明显,两者之间差异显著(P < 0.01或者P < 0.05)?血清抵抗素水平与血清TNF-α?IL-1β?CRP水平和胰腺组织病理评分呈正相关性,血清CRP水平和胰腺组织病理评分也呈正相关性?结论:抵抗素可能与急性胰腺炎的?发生?发展有关,是评估急性胰腺炎严重程度的有价值指标之一?
英文摘要:
      Objective:To investigate the variety of resistin,CRP,TNF-α and IL-1β in the rat of acute pancreatitis. Methods:Forty Sprague-Dawley rats were randomly divided into four groups:control group,sham operation group,AEP group and ANP group. AEP model was induced by intraperitoneal injection of cerulein,and ANP model was established by intraperitoneal injection of L-arginine while the control group didn’t receive any interference and the sham opertation group received same volume injections of 0.9% saline solution. The level of amylase,resistin,CRP,TNF-α and IL-1β were determined in blood serum by using ELISA,and pancreas/body weight ratio was evaluated and pancreatic pathology was recorded. The level of resistin was detected in pancreas by immunohistochemistry. Results:The levels of amylase,pancreas/body weight ratio,pancreatic pathology,resistin,TNF-α,IL-1β and CRP were respectively(4376.70±342.95)U/L,(8.67±1.43)g/kg,5.39±0.26,(10.21±3.34)ng/ml,(184.18±45.24)pg/ml,(194.24±44.81)pg/ml,(3585.85±157.28)ng/ml in AEP group;and were respectively (6750.2±321.81)U/L,(9.33±1.76)g/kg,7.81±0.28,(15.14±3.11)ng/ml,(349.31±94.54)pg/ml,(315.59±37.04)pg/ml,(4345.04±661.53)ng/ml in ANP group. All the indexes were significantly higher than those in the control group and the sham operation group(P < 0.01),the levels of the indexes in ANP group were higher than those in AEP group(P < 0.01 or P < 0.05). Resistin was correlated with the increase of CRP,TNF-α,IL-1β and the pathologic severitity of pancreatitis,respectively,(r=0.711,0.812,0.794,0.812,P < 0.01). The pathologic severitity of pancreatitis was correlated with the level of CRP,TNF-α,IL-1β. Conclusion:The resistin is correlated with the pathogenesis and development of acute pancreatitis,and may be useful to predict the severity and prognosis of acute pancreatitis.
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