大鼠新型隐球菌脑感染的早期MRI表现与病理基础
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An experimental study of cerebral cryptococcus neoformans infection of rats:early MRI appearances and pathologic basis
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    目的:探讨大鼠新型隐球菌脑感染早期MRI表现及其病理基础-方法:将96只Wistar大鼠随机分成免疫抑制感染组(n=40)-正常状态感染组(n=40)-单纯免疫抑制对照组(n=8)-正常对照组(n=8)-感染组侧脑室内注射新型隐球菌菌液-第7-14-21-28天使用1.5T MR对大鼠颅脑进行常规平扫-增强-DWI等多序列检查,定期处死观察病变处病理变化情况-结果:①脑膜强化:第7天MRI扫描可见感染组大鼠脑膜轻度强化,免疫抑制感染组和正常状态感染组大鼠在第7-14-21-28天脑膜强化分别为4-4-3-3和2-1-1-1只;②脑实质病灶:第7-14-21-28天两组大鼠脑实质内病灶分别为1-3-6-6和0-0-1-1只,两组之间在第21-28天存在统计学差异(P=0.04)-③脑室扩大:第21天开始见脑室扩大,免疫抑制感染组与正常状态感染组分别为4只-1只,第28天脑室扩大更明显,两组分别为6只-1只;④动态观察:免疫抑制状态下动态观察组脑内病灶随时间延长而变大,病灶变多,脑室扩大更明显;⑤病理检查:脑膜或脑实质病变处可见隐球菌菌体,局部淋巴细胞聚集,脑实质内可见隐球菌菌落周围胶样团块,局灶性坏死-结论:免疫抑制状态下,大鼠更易发生新型隐球菌脑感染-大鼠新型隐球菌脑感染的MRI早期表现为脑膜脑炎,脑实质内感染在MRI上表现为局限性炎症,胶样假囊,随感染时间延长可见脑室系统的扩大-

    Abstract:

    Objective:To investigate the early MRI appearances and pathologic basis of cryptococcal neoformans infection in rats. Methods:96 Wistar rats were randomly divided into(1)immunocompromised infected group(n=40),(2)normal status infected group(n=40),(3)simple immunosuppression control group(n=8),(4)normal control group(n=8). Infected rats were injected intracerebrally with the suspension of cryptococcus neoformans. At 7,14,21,28 days,the rats were scanned with conventional imaging,enhancement scanning,and DWI,and then were killed to observe the pathological changes of lesions. Results:(1)Meningeal enhancement:At 7 days,MRI showed meningeal enhancement in infected rats. Meningeal enhancement observed in rats of immunocompromised infected group and normal status infected group were 4,4,3,3 and 2,1,1,1 respectively at 14,21,28 days.(2)Brain parenchymal lesions:1,3,6,6 and 0,0,1,1 lesions of the two groups were seen respectively at 7,14,21,28 days. There were significant differences(P=0.04)between the two groups at 21,28 days respectively.(3)Dilated ventricle was observed in 4 immunosuppressive rats and one normal status infected rat at 21 days. More obviously dilated ventricle was seen at 28 days of the two groups,respectively for 6,1 rats.(4)Brain lesions became larger and more with time,in dynamic observed group in rats of immunosuppressive status.(5)Pathological examination:Cryptococcus neoformans were seen on part of meningitis and in the brain parenchyma. The gelatinous pseudocysts around the cryptococcus neoformans,lymphocytic infiltration,focal necrosis were observed with HE and PAS staining. Conclusion:Immunocompromised rats are easier to be infected by cryptococcal neoformans. The earliest finding of cryptococcal neoformans infection in rats on MRI was meningoencephalitis. MRI findings of brain parenchymal infection shows limited inflammation,gelatinous pseudocysts,and dilated ventricle.

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冯〓峰,施裕新,夏淦林,张志勇,王桂龙.大鼠新型隐球菌脑感染的早期MRI表现与病理基础[J].南京医科大学学报(自然科学版),2010,(7):966-970

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  • 收稿日期:2009-12-17
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