文章摘要
罗小军,孔宪炳.Sonic Hedgehog信号通路分子Shh和Smo在肝细胞肝癌中表达与意义[J].南京医科大学学报,2010,(8):1065~1068
Sonic Hedgehog信号通路分子Shh和Smo在肝细胞肝癌中表达与意义
The expression and significance of Shh and Smo in the Sonic Hedgehog signaling pathway in hepatocellular carcinoma
投稿时间:2010-01-19  
DOI:10.7655
中文关键词: Sonic Hedgehog信号通路  肝癌  
英文关键词: Sonic Hedgehog signaling pathway  hepatocellular carcinoma  carcinoma
基金项目:重庆市卫生局科研项目基金(2008-2-40)
作者单位
罗小军 重庆医科大学附属第一医院肝胆外科,重庆〓400016 
孔宪炳 重庆医科大学附属第一医院肝胆外科,重庆〓400016 
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中文摘要:
      目的:探讨Sonic Hedgehog(SHH)信号通路分子Shh和Smo在肝细胞肝癌(Hepatocellular carcinoma,HCC)中的表达及其意义?方法:用RT-PCR方法检测10例HCC组织及相应癌旁组织和肝癌细胞系HepG2?Huh7中Shh?Smo mRNA的表达?采用免疫组化方法检测30例肝癌组织及10例正常肝组织中Shh?Smo蛋白的表达?结果:RT-PCR结果显示HCC组织中Shh?Smo mRNA的表达率显著高于癌旁组织(P < 0.05);Shh?Smo mRNA在Huh7中的表达强度高于HepG2,但两者间无显著性差异(P > 0.05)?免疫组化结果显示Shh?Smo蛋白在HCC组织中阳性率分别为63.3%(19/30)?76.7%(23/30);Smo蛋白的表达与肿瘤大小相关(P < 0.05)?Shh?Smo蛋白在正常肝组织中均无表达?结论:Shh和Smo在肝癌中高表达,表明SHH信号通路可能参与肝癌的发生,为肝癌防治提供新的依据?
英文摘要:
      Objective:To explore the expression and significance of Shh and Smo in Sonic Hedgehog(SHH)signaling pathway in hepatocellular carcinoma(HCC). Methods:The expression of Shh and Smo were detected in 10 fresh HCC tissues and 2 HCC cell lines of HepG2 and Huh7 by using RT-PCR,and also observed in 30 HCC tissue slices and 10 normal liver tissue slices using immunohistochemistry method. Results:The mRNA expressions of Shh and Smo were higher in HCC than in adjacent-tumor tissues(P < 0.05). The mRNA expressions of Shh and Smo were higher in Huh7 cell lines than in HepG2,but no significant difference was observed between two cell lines(P > 0.05). Immunohistochemistry results showed in 30 HCC tissue slices,the positive expression rates of Shh and Smo protein were 63.3%(19/30) and 76.7%(23/30),respectively. The expression of Smo protein was significantly correlated with the tumor size(P < 0.05). Shh and Smo proteins did not expressed in normal liver tissue. Conclusion:The expressions of Shh and Smo were elevated in HCC,which indicated that SHH signaling pathway may involve in human HCC carcinogenesis. The study may be useful for treatment of HCC.
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