文章摘要
吕〓鑫,仇红霞,居颂文,许〓戟,吴雨洁,李建勇.B7H3分子在人髓性白血病细胞株U937和K562上的表达及生物学意义[J].南京医科大学学报,2010,(8):1079~1081
B7H3分子在人髓性白血病细胞株U937和K562上的表达及生物学意义
Expression of B7H3 in human leukemic cell lines of U937 and K562
投稿时间:2010-01-11  
DOI:10.7655
中文关键词: B7H3  人髓性白血病细胞株  细胞生长
英文关键词: B7H3  human leukemic cell lines  proliferation
基金项目:
作者单位
吕〓鑫 南京医科大学第一附属医院血液科,江苏 南京〓210029 
仇红霞 南京医科大学第一附属医院血液科,江苏 南京〓210029 
居颂文 南京医科大学第一附属医院血液科,江苏 南京〓210029 
许〓戟 南京医科大学第一附属医院血液科,江苏 南京〓210029 
吴雨洁 南京医科大学第一附属医院血液科,江苏 南京〓210029 
李建勇 南京医科大学第一附属医院血液科,江苏 南京〓210029 
摘要点击次数: 1477
全文下载次数: 182
中文摘要:
      目的:研究共刺激分子B7H3分子在人髓性白血病细胞株U937和K562上的表达及其生物学意义?方法:应用流式细胞术及RT-PCR法检测B7H3分子在人髓性白血病细胞株U937和K562上的表达;并用鼠抗人B7H3单克隆抗体(mAb)作用于U937和K562细胞株,细胞计数法检测鼠抗人mAb B7H3对U937和K562细胞株生长的影响?结果:流式细胞术分析显示U937和K562细胞株均表达B7H3膜蛋白,RT-PCR检测到U937和K562细胞有B7H3 mRNA产物;鼠抗人mAb B7H3对U937和K562细胞株有抑制生长的作用?结论:U937和K562细胞株组成性表达B7H3分子;鼠抗人mAb B7H3与U937和K562细胞表面的B7H3分子交联后可以抑制白血病细胞的生长?
英文摘要:
      Objective:To study the expression and function of B7H3 in human myeloid leukemic cell lines,U937 and K562. Methods:The protein and mRNA expression levels of B7H3 were detected by FACS and RT-PCR methods,respectively. The effect of B7H3 in human leukemic cell lines was studied by B7H3 mAb inhibiton assay. Cell proliferation was determined by cell counting. Results:B7H3 expressed in both U937 and K562 cell lines. B7H3 mAb could inhibit cell proliferation of those two cell lines. Conclusion:B7H3 is constitutive expression in human myeloid leukemia cell lines,U937 and K562. B7H3 mAb can conjugate with B7H3 expressed in cell surface,which plays a critical role in the survival and growth of leukemia cells.
查看全文   查看/发表评论  下载PDF阅读器
关闭