文章摘要
周 峰,张红闯,卢士强,李凌云,朱 玲,姚 堃.口腔鳞状细胞癌与人类疱疹病毒6型感染的相关性研究[J].南京医科大学学报,2010,(10):1379~1382
口腔鳞状细胞癌与人类疱疹病毒6型感染的相关性研究
Human herpesvirus 6 in oral squamous cell carcinoma
  
DOI:10.7655
中文关键词: 口腔鳞状细胞癌  人类疱疹病毒6型  动物模型
英文关键词: oral squamous cell carcinom (OSCC)  human herpesvirus 6 (HHV-6)  animal model
基金项目:国家自然科学基金项目(30771961,30972784)
作者单位
周 峰 南京医科大学微生物与免疫学系,江苏 南京 210029 
张红闯 南京医科大学附属口腔医院,江苏 南京 210029 
卢士强 南京医科大学微生物与免疫学系,江苏 南京 210029 
李凌云 南京医科大学微生物与免疫学系,江苏 南京 210029 
朱 玲 南京医科大学附属口腔医院,江苏 南京 210029 
姚 堃 南京医科大学微生物与免疫学系,江苏 南京 210029 
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中文摘要:
      目的:研究人类疱疹病毒6型(HHV-6)感染与口腔鳞癌的相关性?方法:用PCR和间接免疫荧光方法分别检测口腔鳞癌患者和健康志愿者唾液HHV-6 DNA和血清抗HHV-6 IgG抗体?免疫组化法检测口腔鳞癌组织及癌旁组织标本中HHV-6糖蛋白B(glycoprotein B,gB)抗原?结果:118份口腔鳞癌唾液标本中PCR阳性率为65.3%,高于正常人群的40%(P < 0.05)?HHV-6感染与患者病历资料无显著相关性?18份口腔鳞癌患者和20份健康人血清中HHV-6 IgG抗体的几何平均滴度分别为1∶102.17和1∶101.50(P < 0.05)?免疫组化法检测,11例口腔鳞癌标本中6例出现的阳性结果,胞质内出现弥散性的黄色颗粒,在癌巢内特异性浓集,而间质为阴性?8例相应癌旁组织中3例出现阳性染色结果,但阳性强度较癌组织弱且弥散?1例gB组化阳性肿瘤组织细胞经分离后接种裸鼠,移植瘤组织也检测到HHV-6抗原?结论:本研究说明HHV-6与口腔鳞癌的发生存在相关性,但不与患者临床资料分布相关?本研究亦提示荷瘤裸鼠作为HHV-6动物模型的可能性?
英文摘要:
      Objective:To investigate the relationship between oral squamous cell carcinoma (OSCC) and human herpesvirus 6 (HHV-6) infection. Methods:HHV-6 DNA in saliva from patients of OSCC and from health control people was amplified by nest PCR which was HHV-6 specific. Serum anti-HHV-6 IgG titer was tested by anti-HHV-6 IFA kit. HHV-6 glycoprotein B(gB) was detected by immunohistochemistry in OSCC tissues and peritumoral tissues,respectively. Results:The detectable rate of HHV-6 DNA in patients of OSCC is 65.3%,which was much higher than 40% of health control group. But there was no significant difference in clinicopathologic and background variables of OSCC between the PCR positive and PCR negative groups. The geometric mean titer of anti-HHV-6 IgG was significantly higher in the OSCC patients (1∶102.17) than in the health controls (1∶101.50). HHV-6 gB was detected in 6 out of 11 tissues of OSCC and in 3 out of 8 peritumoral tissues. The gB was condensated in tumour cells while was either negative or much lower expression in the intertumor issues. We isolated and cultured tumor cells from one tissue of OSCC which was positive for HHV-6 gB. And then the nude mouse was inoculated with those tumor cells. We could also detected HHV-6 gB in tumor tissues from the mouse. Conclusion:The results demonstrated the frequent presence of HHV-6 in OSCC,although which has no association with clinicopathologic and background variables of patients. An animal model which carry HHV-6 antigen were established and could be useful for understanding the role that the HHV-6 really play in the genesis and treatment of OSCC which is HHV-6 positive.
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