文章摘要
石学桂,赵宇岚,王秋杰.血清淀粉样蛋白A通过NFκB途径诱导人脐静脉内皮细胞E-选择素的表达[J].南京医科大学学报,2010,(10):1393~1396
血清淀粉样蛋白A通过NFκB途径诱导人脐静脉内皮细胞E-选择素的表达
Serum amyloid A induces E-selectin expression through NFκB pathway in human umbilical vein cells
投稿时间:2010-03-05  
DOI:10.7655
中文关键词: 人脐静脉内皮细胞  血清淀粉样蛋白A  E-选择素
英文关键词: HUVEC  SAA  E-Selectin
基金项目:国家自然科学基金(30800401);上海高校选拔培养优秀青年教师科研专项基金(79301636)
作者单位
石学桂 华东师范大学科学与技术跨学科高等研究院,上海 200062 
赵宇岚 华东师范大学科学与技术跨学科高等研究院,上海 200062 
王秋杰 新加坡国立大学医学院儿科,新加坡 119074 
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中文摘要:
      目的:研究血清淀粉样蛋白A(serum amyloid A,SAA)对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)E-选择素(E-Selectin)表达的影响及可能的信号途径?方法:用不同浓度的SAA作用HUVEC细胞,通过实时荧光定量聚合酶链反应的方法检测E-Selectin mRNA的表达,用酶联免疫吸附实验的方法检测蛋白的表达?结果:20 μg/ml重组人SAA蛋白的刺激显著诱导了HUVEC的E-Selectin的转录,蛋白表达及分泌?用特异性的核因子NFκB抑制剂BAY-11-7082可以阻断SAA对E-Selectin的诱导作用?凝胶迁移实验的结果显示,SAA作用直接活化NFκB?结论:SAA通过NFκB途径诱导HUVEC中E-Selectin的表达?
英文摘要:
      Objective:To investigate the effects of serum amyloid A(SAA) on the expression of E-Selectin in human umbilical vein endothelial cells(HUVECs) and the potential signaling pathway. Methods:HUVECs were incubated with recombinant human SAA (0-40 μg/ml). The transcriptional level of E-selectin was assayed by real-time PCR. The protein secretion was determined by ELISA assay. Results:SAA (20 μg/ml) markedly induced E-selectin expression of mRNA and protein,and promoted E-selectin secreting in HUVECs. Pretreatment with a specific NFκB inhibitor,Bay-11-7082,could block the SAA-dependent E-selectin induction. Furthermore,electrophoretic mobility shift assay (EMSA) results showed that SAA could directly induce the activation of NFκB. Conclusion:SAA induces E-selectin expression through the NFκB pathway in HUVECs.
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