文章摘要
余 海,凌亭生,施瑞华.TESTIN基因在人食管鳞癌及其癌旁组织中的表达及意义[J].南京医科大学学报,2010,(10):1408~1412
TESTIN基因在人食管鳞癌及其癌旁组织中的表达及意义
Detection of TESTIN in human esophageal squamous cancer tissues
投稿时间:2010-03-26  
DOI:10.7655
中文关键词: 食管肿瘤  鳞状细胞癌  抑癌基因TESTIN
英文关键词: esophageal neoplasms  squamous cell carcinoma  suppressor gene TESTIN
基金项目:江苏省卫生厅科教兴卫工程重点人才资助项目(200728)
作者单位
余 海 南京医科大学第一附属医院消化科,江苏 南京 210029 
凌亭生 南京医科大学第一附属医院消化科,江苏 南京 210029 
施瑞华 南京医科大学第一附属医院消化科,江苏 南京 210029 
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中文摘要:
      目的:研究TESTIN在食管鳞状细胞癌及癌旁组织中的表达及临床意义?方法:应用免疫组织化学S-P法和Western blot技术以及实时荧光定量PCR(FQ-RT-PCR)技术检测65例配对人食管鳞癌组织和癌旁正常组织中TESTIN表达的差异,并分析TESTIN的表达与患者临床病理特征的关系?结果:65例配对人食管鳞癌组织中,TESTIN mRNA的表达量显著低于癌旁正常组织(P < 0.05);免疫组化结果显示TESTIN在36.9%(24/65)的食管鳞癌组织中呈阳性,而在正常组织中有87.7%(57/65)阳性(P < 0.01);Western blot结果显示69.2%(45/65)的食管鳞癌组织TESTIN蛋白表达量比对应癌旁正常组织下降?统计结果分析发现,TESTIN的表达与食管鳞癌分化程度有关,而与性别?年龄?肿瘤大小?TNM分期?淋巴结转移关系无统计学意义?结论:TESTIN基因的低表达在食管鳞癌的发生?发展过程中起一定的作用,可能是一种潜在的抑癌基因,并且可能成为食管鳞癌诊断和判断其恶性程度的一个的指标?
英文摘要:
      Objective:To the investigate the expression of TESTIN in human esophageal squamous cancer tissues,and discuss its value in the development of esophageal squamous cell cancer(ESCC) . Methods:The expression of TESTIN in 65 matched-pairs of ESCC tissues were detected by immunohistochemical staining,western blot,and FQ-RT-PCR methods. The correlations of TESTIN to clinicopathologic features of ESCC were analyzed. Results:The mRNA level of TESTIN was down-regulated in ESCC (P < 0.05).The positive rate of TESTIN protein was significantly lower in ESCC tissues than that in nomal tissues (36.9% vs 87.7 %,P < 0.01). The protein level of TESTIN was down-regulated in 45 (69.2%) of 65 cancers as compared with corresponding normal tissues. TESTIN expression was positively correlated with the differentiation of ESCC. Meanwhile,differences in gender,age,tumor size,TNM stage and lymph node metastasis were not significant. Conclusion:The decreased expression of TESTIN gene may play an importmant role in the development of esophageal squamous cancer. And thus TESTIN gene might be a novel candidate of tumor-suppressor. It may be an objective marker for prognostic factor and malignant level for ESCC.
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