文章摘要
王艳萍,洪 琴,郭 凯,池 霞,郭锡熔,童梅玲.多氯联苯暴露对斑马鱼胚胎发育的毒性效应[J].南京医科大学学报,2010,(11):1537~1541
多氯联苯暴露对斑马鱼胚胎发育的毒性效应
Toxicity effects of polychlorinated biphenyls on development of zebrafish embryo
投稿时间:2010-04-22  
DOI:10.7655
中文关键词: 多氯联苯  斑马鱼  胚胎发育  毒性效应
英文关键词: polychlorinated biphenyls  zebrafish  development of embryos  toxic effect
基金项目:南京医科大学科技发展基金(09NJMUM086)
作者单位
王艳萍 南京医科大学儿科研究所,江苏 南京 210029 
洪 琴 南京医科大学附属南京市妇幼保健院,江苏 南京 210004 
郭 凯 南京医科大学儿科研究所,江苏 南京 210029 
池 霞 南京医科大学附属南京市妇幼保健院,江苏 南京 210004 
郭锡熔 南京医科大学附属南京市妇幼保健院,江苏 南京 210004 
童梅玲 南京医科大学附属南京市妇幼保健院,江苏 南京 210004 
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中文摘要:
      目的:探讨环境有机污染物多氯联苯(PCBs)对斑马鱼胚胎发育的影响?方法:以斑马鱼为模式生物,配制3.000?2.000?1.000?0.500?0.250?0.125?0.050 mg/L 7个不同浓度的PCB1254对斑马鱼胚胎进行暴露处理,观察PCB1254暴露对斑马鱼胚胎发育形态及孵化率?死亡率的影响?结果:①中?高浓度(0.25 mg/L以上)的PCB1254暴露对斑马鱼胚胎发育具有一定的致死和致畸效应,随着PCB1254浓度的升高,斑马鱼受精卵的孵化率明显降低,而致畸率?死亡率明显升高,存在着明显的剂量-效应关系;② PCB1254暴露造成的斑马鱼胚胎发育畸形主要表现为脊椎弯曲和心包水肿;③低浓度(0.125 mg/L以下)PCB1254暴露对斑马鱼胚胎发育无明显影响; ④PCB1254暴露96 h对斑马鱼半致死浓度LC50为1.082 mg/L,95%可信区间范围为0.917~1.327 mg/L?结论:不同浓度的PCB1254暴露对斑马鱼胚胎发育具有不同的致死和致畸效应,提示必须加强对环境有机污染物的监测和控制?
英文摘要:
      Objective:To study the effects of polychlorinated biphenyls(PCBs) on the development of zebrafish embryo. Methods: We studied the influences of different concentrations (3.000, 2.000, 1.000, 0.500, 0.250, 0.125, 0.050 mg/L) of PCB1254 on morphological development, mortality rate and percentage of hatching using zebrafish as the model animal. Results: ①PCB1254 got teratogenic and mortality effect on zebrafish both at high and moderate concentration (above 0.25 mg/L). With the concentration increasing, hatching rate of zebrafish egg decreased while the mortality rate and the teratogenic rate risd obviously, which indicated that there was an obvious dose-depending effect; ②Deformities of zebrafish embryo exposed to PCB1254 mainly manifested spinal curvature and pericardialites; ③No effect was observed with the concentration of PCB1254 below 0.125 mg/L on the zebrafish; ④The 96 h LC50 of PCB1254 on the zebrafish was 1.082 mg/L, with 95% confidence interval ranging from 0.917 to 1.327 mg/L. Conclusion:Teratogenic and mortality effects are obersved on the development of zebrafish embryo exposed to different concentrations of PCB1254, which suggests the value of monitoring organic contaminant.
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