文章摘要
韦翠萍,邱秀芹,顾振纶.沙苑子黄酮对人乳腺癌细胞MCF-7增殖抑制?诱导凋亡及上调p53表达?下调NF-κB?c-Myc表达作用[J].南京医科大学学报,2010,(11):1556~1559
沙苑子黄酮对人乳腺癌细胞MCF-7增殖抑制?诱导凋亡及上调p53表达?下调NF-κB?c-Myc表达作用
FAC inhibits proliferation,induces apoptosis of human breast cancer MCF-7,increases expression of p53 and decrease expression of c-Myc and NF-κB
投稿时间:2010-05-28  
DOI:10.7655
中文关键词: 沙苑子黄酮  MCF-7  凋亡  NF-κB  c-Myc  p53
英文关键词: flavonoids of Astragalus complanatus(FAC)  MCF-7  apoptosis  NF-кB  c-Myc  p53
基金项目:
作者单位
韦翠萍 苏州卫生职业技术学院药理教研室,江苏 苏州 215009 
邱秀芹 苏州卫生职业技术学院生化教研室,江苏 苏州 215009 
顾振纶 苏州大学药理教研室,江苏 苏州 215123 
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中文摘要:
      目的: 研究沙苑子黄酮(flavonoids of Astragalws complanatus,FAC)抑制人乳腺癌细胞MCF-7增殖?诱导其凋亡以及FAC对核转录因子κB(nuclear factor-κB,NF-κB)?c-Myc?p53表达的影响?方法:以不同浓度FAC(0.0625?0.125?0.25?0.5 mg/ml) 处理MCF-7细胞12?24?48?72 h后,MTT法测定细胞增殖情况;用流式细胞分析术检测FAC诱导凋亡作用;用荧光免疫法?Western blot法检测凋亡相关蛋白的表达?结果:MTT法显示,不同浓度FAC作用MCF-7细胞24?48?72 h后对细胞增殖具有明显抑制作用,与阴性对照组相比差异具有显著性(P < 0.05);抑瘤率与FAC剂量?作用时间呈依赖性?流式细胞分析显示,FAC能明显诱导MCF-7细胞凋亡,0.0625?0.125?0.25?0.5 mg/ml FAC作用细胞36 h后,凋亡率分别为6.5%?18.3%?49.6%和57.9%?荧光免疫法及Western blot法显示,FAC作用后MCF-7中c-Myc与NF-κB表达明显减弱;而p53表达显著增强,并向细胞核内聚集?结论:FAC对MCF-7具有抑制增殖和诱导凋亡作用;其作用可能与其下调NF-кB?c-Myc表达?上调p53表达有关?
英文摘要:
      Objective: To research the effects of flavonoids of Astragalus complanatus(FAC) on proliferation,apoptosis and expressions of p53,c-My and NF-кB in human breast cancer MCF-7 cells. Methods:The inhibit rate of MCF-7 cells treated by different concentration of FAC was studied by MTT at different times. The apoptosis rate of MCF-7 cell was essayed by flow cytometry after treated with effective concentration of FAC and time points according to MTT. The expressions of p53, c-Myc and NF-кB were quantified by fluorescence immunoassay and Western blot. Results:According to MTT,0.0625,0.125,0,25,0.5 mg/ml of FAC treated for 24 h, 48 h,72 h markedly inhibited proliferation of MCF-7(P < 0.05,vs control). According to flow cytometry, MCF-7 was induced to apoptosis by FAC. After 72 h treatment with FAC(0.0625,0.125,0.25,0.5 mg/ml), the apoptosis rates were 6.5%,18.3%,49.6% and 57.9%,respectively. The expression of p53 was up-regulated, while c-Myc and NF-κB were markedly reduced detected by fluorescence immunoassay and Western blot. Conclusion:FAC could inhibit proliferation and induce apoptosis of MCF-7. Its role may be related to decreased expression of NF-кB and c-Myc, increased expression of p53.
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