文章摘要
潘寅兵,王 娴,马绍磊,周志强,杨建军.活性氧介导大剂量氯胺酮致原代培养皮层神经元凋亡[J].南京医科大学学报,2010,(11):1560~1563
活性氧介导大剂量氯胺酮致原代培养皮层神经元凋亡
ROS mediates large dose of ketamine-induced apoptosis in cultured primary neuron
投稿时间:2010-06-02  
DOI:10.7655
中文关键词: ROS  氯胺酮  凋亡  大鼠
英文关键词: ROS  ketamine  apoptosis  rat
基金项目:国家自然科学基金资助(30872424)
作者单位
潘寅兵 南京医科大学第一附属医院麻醉科,江苏 南京 210029 
王 娴 南京军区总医院麻醉科,江苏 南京 210002 
马绍磊 徐州市第三人民医院急诊内科,江苏 徐州 221005 
周志强 南京军区总医院麻醉科,江苏 南京 210002 
杨建军 南京军区总医院麻醉科,江苏 南京 210002 
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中文摘要:
      目的:观察活性氧(ROS)是否参与大剂量氯胺酮致原代培养皮层神经元凋亡?方法:单纯培养基(C组)?1 mmol/L氯胺酮(K组)?1 mmol/L氯胺酮复合2.5 μmol/L超氧化物歧化酶类似物M40403(M组)分别作用于体外原代培养第6天的神经元24 h,检测神经元ROS生成?凋亡细胞百分比及促凋亡蛋白Bax表达?结果:与C组相比,K组神经元ROS生成?凋亡细胞百分比?Bax表达分别是其1.7?4.2及2.0倍(P < 0.05);与C组相比,M组神经元ROS生成?凋亡细胞百分比?Bax表达差异无统计学意义?结论: ROS介导大剂量氯胺酮致原代培养皮层神经元凋亡?
英文摘要:
      Objective: To observe whether reactive oxygen species(ROS) is involved in large dose ketamine-induced apoptosis on cultured primary neuron. Methods: Neuronal culture media (C group), 1 mmol/L ketamine (K group) or 1 mmol/L ketamine combined with 2.5 μmol/L superoxide dismutase mimetic M40403 (M group) was applied to primary neuron at day 6 for 24 h. Intraneuronal ROS production, the percentage of apoptotic cells and pro-apoptotic protein Bax expression were measured. Results: ROS production, the percentage of apoptotic cells and Bax expression in K group were 1.7, 4.2, and 2.0-fold of those in C group, respectively(all P < 0.05). Compared with C group, M group had no statistically significant changes in ROS production, the percentage of apoptotic cells and Bax expression (all P > 0.05). Conclusion: ROS could mediate the apoptosis induced by large dose ketamine in cultured primary neuron.
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