文章摘要
杨小冬,黄 平,王 锋,杨 力,徐泽宽,李小腾.生长激素对结肠癌肿瘤细胞体外影响[J].南京医科大学学报,2010,(11):1604~1607
生长激素对结肠癌肿瘤细胞体外影响
Effects of growth hormone on colon cancer cell (Lovo cell) in vitro
投稿时间:2010-05-25  
DOI:10.7655
中文关键词: 结直肠癌  生长激素  细胞周期  细胞凋亡
英文关键词: colorectal cancer  growth hormone  cell cycle  cell apoptosis
基金项目:
作者单位
杨小冬 南京医科大学第一附属医院普外科, 江苏 南京 210029 
黄 平 南京医科大学第一附属医院普外科, 江苏 南京 210029 
王 锋 南京医科大学第一附属医院普外科, 江苏 南京 210029 
杨 力 南京医科大学第一附属医院普外科, 江苏 南京 210029 
徐泽宽 南京医科大学第一附属医院普外科, 江苏 南京 210029 
李小腾 南京医科大学第一附属医院普外科, 江苏 南京 210029 
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中文摘要:
      目的: 检测生长激素对体外培养的结直肠癌细胞的影响,并探讨其作用机制?方法:结肠癌细胞株Lovo细胞随机分组,加或不加不同浓度的生长激素孵育不同时间后,以MTT法检测细胞增殖情况,流式细胞术分析细胞周期,Annexin V/PI双染色法检测细胞凋亡情况?结果:MTT法测定表明,生长激素对Lovo细胞有明显的促进增殖效应,12~24 h起效,48 h时达高峰,生长激素达到一定浓度后,其促增殖作用不再增加?流式细胞仪检测生长激素对结肠癌细胞的影响显示:浓度为200 ng/ml的生长激素作用Lovo细胞24 h后,G0/G1细胞比例下降,S期细胞比例明显上升,G2/M期细胞比例稍上升,凋亡指数无明显变化(P=0.187);72 h时, G0/G1期细胞下降更为明显,S期细胞比例上升亦更为明显,G2/M期细胞上升明显,凋亡细胞比例明显增高(P < 0.01)?结论:生长激素对Lovo细胞有明显的促增殖作用,并有受体饱和效应?该作用是通过改变细胞周期分布,促进G0/G1期细胞向S期?G2/M期细胞转变实现的?
英文摘要:
      Objective: To characterize the effects of growth hormone (GH) on human colon cancer in vitro. Methods: Lovo cells were divided into control group and experimental groups and treated with different concentration of GH. MTT assay was used to determine the growth of the cells. The cell cycle and cell apoptosis of Lovo cells were detected by flow cytometer with PI and annexin V/PI double staining. Results: GH promoted the proliferation of Lovo cells, and the effects began at 12~24 h and peaked at 48 h. GH got the best effect on proliferation of Lovo cells when the concentration reached 200 ng/ml. After Lovo cells were treated by 200 ng/ml growth hormone for 24 h, the rate of G0/G1 stage decreased significantly, the rate of S stage increased significantly and the rate of G2/M stage increased slightly. For 72 h, the rate of G0/G1 stage decreased more significantly, the rate of S stage increased more significantly and the rate of G2/M stage increased significantly,the percentage of apoptotic cells also increased significantly. Conclusion: GH is a powerful promoter on proliferation of Lovo cells growth in culture. The effect of growth hormone manifesed a dose-dependent manner, and the saturation effect of growth hormone occurred at the concentration of 200 ng/ml, and its mechanism could be performed by promotion the cells G0/G1 stage to S and G2/M stage.
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