文章摘要
李文光,吴 优,倪 俭,蔡 波,张跃平.前列腺癌组织中PSMA基因及蛋白的表达[J].南京医科大学学报,2010,(11):1608~1611
前列腺癌组织中PSMA基因及蛋白的表达
Expression of PSMA gene and protein in prostate cancer
投稿时间:2010-06-10  
DOI:10.7655
中文关键词: 前列腺癌  前列腺特异性膜抗原  基因  蛋白
英文关键词: prostate cancer  PMSA  gene  protein
基金项目:
作者单位
李文光 南通大学附属医院泌尿外科,江苏 南通 226001 
吴 优 南通大学附属医院泌尿外科,江苏 南通 226001 
倪 俭 南通大学附属医院泌尿外科,江苏 南通 226001 
蔡 波 南通大学附属医院泌尿外科,江苏 南通 226001 
张跃平 南通大学附属医院泌尿外科,江苏 南通 226001 
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中文摘要:
      目的: 检测前列腺特异性膜抗原(prostate specific membrane antigen,PSMA)基因及蛋白在前列腺癌组织中的表达?方法:分别采用半定量RT-PCR法及免疫组织化学法检测52例前列腺癌组织及35例前列腺增生组织中PSMA基因及蛋白的表达情况?结果:①PSMA在前列腺癌及前列腺增生组织中表达阳性率分别为84.6%(44/52)及68.6%(24/35),虽然前列腺癌组织中PSMA表达阳性率高于前列腺增生组织,但两组阳性率间差异无统计学意义(P > 0.05)?PSMA mRNA半定量结果在两组间存在差异,癌组织中PSMA的表达量明显高于增生组织中的表达(P < 0.05)?②前列腺癌组织中PSMA蛋白表达总阳性率高达94.2%,明显高于增生组织中的阳性率(65.7%),且以++~+++居多?结论:PSMA基因及蛋白的表达在前列腺癌组织均高于前列腺增生组织,提示其可作为前列腺癌诊断指标?
英文摘要:
      Objective: To investigate the expression of prostate specific membrane antigen(PSMA) gene and protein in prostate cancer. Method:Expression of PSMA gene and protein were assayed by semiquantitive RT-PCR and immunohistochemistry method in 52 cases of prostate cancer and 35 cases of benign prostatic hyperplasia tissues. Result:①The positive rates of PSMA gene in the tissues of prostate cancer and benign prostatic hyperplasia were 84.6% and 68.6% respectively. Althought there was no significant difference between the two groups, the semiquantitive results showed that the expression level of PSMA gene in prostate cancer was significantly higher than in benign prostatic hyperplasia tissues. ②The positive expression rate of PSMA protein was significantly higher in prostate cancer tissue(94.2%) than that of in benign prostatic hyperplasia tissues(65.7%). Conclusion:The expression levels of PSMA gene and protein were higher in prostate cancer tissues than those of benign prostatic hyperplasia tissues, suggesting that PSMA may be a potential marker for prostate cancer.
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