文章摘要
范 磊,朱丹霞,董华洁,吴雨洁,乔 纯,王 荣,徐 卫,李建勇.抗凋亡基因XIAP在慢性淋巴细胞白血病中的异常表达及其临床意义[J].南京医科大学学报,2010,(12):1683~1686
抗凋亡基因XIAP在慢性淋巴细胞白血病中的异常表达及其临床意义
Abnormal expression of anti-apoptotic gene XIAP in chronic lymphocytic leukemia and its clinical significance
投稿时间:2010-10-27  
DOI:10.7655
中文关键词: 凋亡  慢性淋巴细胞白血病  XIAP  IgVH突变  CD38
英文关键词: apoptosis  chronic lymphocytic leukemia  XIAP  IgVH mutation status  CD38
基金项目:国家自然科学基金资助(30971295);江苏省卫生厅医学科研课题资助(H200703)
作者单位
范 磊 南京医科大学第一附属医院血液科 ,江苏 南京 210029 
朱丹霞 南京医科大学第一附属医院血液科 ,江苏 南京 210029 
董华洁 南京医科大学第一附属医院血液科 ,江苏 南京 210029 
吴雨洁 南京医科大学第一附属医院血液科 ,江苏 南京 210029 
乔 纯 南京医科大学第一附属医院血液科 ,江苏 南京 210029 
王 荣 南京医科大学第一附属医院血液科 ,江苏 南京 210029 
徐 卫 南京医科大学第一附属医院血液科 ,江苏 南京 210029 
李建勇 南京医科大学第一附属医院血液科 ,江苏 南京 210029 
摘要点击次数: 1229
全文下载次数: 91
中文摘要:
      目的: 慢性淋巴细胞白血病(chronic lymphoctyic leukemia,CLL)存在多种因素导致的细胞凋亡受抑,本文主要探讨抗凋亡基因XIAP在慢性淋巴细胞白血病中的表达,并深入分析其与IgVH突变?CD38等临床重要预后指标的相关性?方法:本研究采用SyberGreen法实时定量PCR(qPCR)技术检测37例CLL患者肿瘤细胞中抗凋亡基因XIAP的mRNA表达水平,同时利用28例年龄匹配的正常人作为阴性对照,分析其临床意义?结果:经过qPCR检测正常对照组XIAP表达中位数为0.04040(95% CI:0.01610~0.06471),CLL组XIAP表达为0.07502(0.04383~0.1062),两者具有显著统计学差异(P=0.0025);亚组分析发现耐药组XIAP表达为0.08295(0.02575~0.1402),药物敏感组为0.07020(0.03042~0.1100),耐药组XIAP表达高于药物敏感组,但是未达到统计学差异(P=0.3978)?进一步分析显示XIAP mRNA表达水平和IgVH突变状态和CD38表达相关:IgVH无突变和CD38高表达的CLL患者XIAP表达显著升高(P值分别为0.0265和0.0388)?结论:利用qPCR技术发现抗凋亡基因XIAP在CLL患者中存在异常高表达,耐药组有高于药物敏感组的趋势,并且与CLL临床预后因素IgVH突变状态和CD38表达密切相关,提示其可能成为预测CLL预后的新指标?
英文摘要:
      Objective: Several mechanisms were found in the chronic lymphocytic leukemia (CLL) cell apoptosis-resistant process. The objective of this study was to investigate expression of anti-apoptotic gene XIAP in CLL patients and its clinical association with established prognostic factors(IgVH mutation status and CD38 expression). Methods: A total number of 37 CLL patients and 28 age-matched normal control were enrolled in this study. XIAP mRNA expression was detected by SyberGreen-based real-time PCR, clinical relationship was analyzed by GraphPad Prism software. Results:XIAP median expression was 0.04040(95% CI∶0.01610-0.06471) in CLL group compared to 0.07502(95% CI:0.04383-0.1062) in normal control group (P=0.0025); further sub-groups analysis implicated that XIAP expression was higher in drug-resistant group than that in drug-sensitive without statistical difference(P=0.3978), IgVH non-mutated status and positive CD38 expression were associated with higher XIAP expression. Conclusion:Abnormal XIAP mRNA expression as found in CLL patients with significant correlation with established prognostic factors and potential role in drug resistance, and XIAP may become a new supplement of CLL prognostic markers.
查看全文   查看/发表评论  下载PDF阅读器
关闭