文章摘要
刘学文,蔡爱民,田步先,韩 锟,张雪杰.NF-κB p65在癫大鼠海马中的激活及W-7的保护作用[J].南京医科大学学报,2010,(12):1691~1695
NF-κB p65在癫大鼠海马中的激活及W-7的保护作用
Study on activation of NF-κB p65 in rat hippocampal formation after experimental seizure and neuroprotective effects of W-7
  
DOI:10.7655
中文关键词: NF-κBp65  戊四唑  癫■  W-7
英文关键词: NF-κB p65, PTZ, epilepsy, W-7
基金项目:辽宁省科技厅博士科研启动基金资助(20091049)
作者单位
刘学文 辽宁医学院附属第一医院神经内科, 辽宁 锦州 121001 
蔡爱民 辽宁医学院附属第一医院神经内科, 辽宁 锦州 121001 
田步先 辽宁医学院附属第一医院神经内科, 辽宁 锦州 121001 
韩 锟 辽宁医学院附属第一医院神经内科, 辽宁 锦州 121001 
张雪杰 辽宁医学院附属第一医院神经内科, 辽宁 锦州 121001 
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中文摘要:
      目的: 探讨核转录因子(NF-κB)p65在癫■大鼠海马结构中的激活及其意义,并观察钙调蛋白抑制剂W-7的干预作用?方法:健康SD大鼠58只,随机分为5组,对照组?戊四唑(PTZ)组和三个剂量的W-7组,通过行为学观察探讨W-7的抗癫■作用,应用免疫组织化学方法和免疫印迹方法检测海马组织NF-κBp65蛋白表达的变化?结果:同对照组比较,3个剂量的W-7大鼠发作程度明显减轻,差异有显著性(P < 0.01);免疫组织化学染色和免疫印记实验显示,PTZ组NF-κBp65蛋白表达明显增强(P < 0.01),3个剂量的W-7组表达明显减弱,其差异均具有显著性意义(P < 0.05,P < 0.01)?结论:PTZ导致癫■大鼠脑组织中NF-κBp65蛋白表达增高?W-7对PTZ诱导的癫■大鼠具有保护作用,其作用是通过抑制NF-κBp65的蛋白表达实现的?
英文摘要:
      Objective: To explore the meaning of activation of NF-κBp65 in rats hippocampal formation and the protective effects and mechanisms of calmodulin inhibitor W-7 on PTZ-induced rats. Methods:Fifty-eight healthy adult Sprague-Dawley rats were randomly divided into 5 groups: control group(i.p.NS), PTZ group(i.p.PTZ 65 mg/kg) and W-7 preteated groups (i.p.W-7 25 μmol/kg, 50 μmol/kg, 100 μmol/kg, followed by i.p.PTZ65mg/kg after 10min of W-7 treatment). Behaviors of rats were observed according to the criteria of Racine. The expression of NF-κBp65 in the hippoacampus of rats as examined by means of immunohistochemistry and Western blotting analysis. Results:Immunohistochemical staining and Western blotting analysis showed that NF-κBp65 was expressed in the hippocampus of all groups. Compared with control group, the expression of NF-κB p65 was increased in PTZ group(P < 0.01), while it was decreased in W-7 groups(P < 0.05,P<0.01). Conclusion:The expression of NF-κB p65 is increased in the hippocampus of PTZ-induced rats. W-7 has protective effects on PTZ-induced rats by depressing expression of NF-κB p65.
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