文章摘要
马孝甜,葛志平,孙丽洲.子痫前期内质网应激相关蛋白CHOP/GADD153的表达及其意义[J].南京医科大学学报,2011,(1):49~52
子痫前期内质网应激相关蛋白CHOP/GADD153的表达及其意义
Expression and significance of CHOP/GADD153 related ERS in preeclampsia
投稿时间:2010-07-23  
DOI:10.7655
中文关键词: 子痫前期  内质网应激  凋亡  CHOP/GADD153
英文关键词: preeclampsia  ER stress  apoptosis  CHOP/GADD153
基金项目:国家自然科学基金(81070511);江苏省自然科学基金(21010583)资助
作者单位
马孝甜 南京医科大学第一附属医院妇产科,江苏 南京 210029 
葛志平 南京医科大学第一附属医院妇产科,江苏 南京 210029 
孙丽洲 南京医科大学第一附属医院妇产科,江苏 南京 210029 
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中文摘要:
      目的:研究内质网应激相关蛋白CHOP/GADD153在子痫前期患者胎盘组织中的表达及其意义?方法:选择2009年6月~2010年6月在南京医科大学第一附属医院产科住院分娩的子痫前期患者30例(子痫前期组),以同期正常孕妇35例为对照组?采用RT-PCR技术?蛋白印迹(western-blot)法和免疫组化检测两组孕妇胎盘组织中CHOP/GADD153的mRNA与蛋白表达水平差异?结果:子痫前期组患者胎盘组织中CHOP/GADD153的mRNA与蛋白表达水平均显著高于相应孕周正常妊娠组?结论:CHOP/GADD153表达的增高与子痫前期的发病相关?
英文摘要:
      Objective:To investigate the expression and significance of C/EBP homologous protein/growth-arrest and DNA damage inducible gene 153(CHOP/GADD153) in preeclampsia. Methods: Semiquantitative RT-PCR,immunohistochemistry and Western-blot were used to investigate the expression of CHOP/GADD153 mRNA and protein levels in placenta of 30 women with preeclampsia and 35 normal pregnant women. Results: ①The expression of CHOP/GADD153 mRNA was significantly higher in preeclampsia group(13.416 ± 1.516) than in the normal pregnant group(2.615 ± 0.659,P < 0.01). ②The mean density of CHOP/GADD153 in preeclampsia placenta(0.156 ± 0.008) was significantly higher than that in the th normal pregnant women group(0.143 ± 0.009,P < 0.01). ③The expression of CHOP/GADD153 protein levels was significantly higher in preeclampsia group(16.210 ± 1.217)than in the normal pregnant group(2.710 ± 0.799,P < 0.01). Conclusion:Up regulation CHOP/GADD153 mRNA and excess expression of CHOP/GADD153 protein levels in placenta may be involved in the pathophysiological processes of preeclampsia.
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