文章摘要
李卫星,孙重期,杜 军.细胞凋亡在人乳腺癌细胞拟态血管形成中的作用[J].南京医科大学学报,2011,(2):179~184
细胞凋亡在人乳腺癌细胞拟态血管形成中的作用
The involvement of apoptosis in vasculogenic mimicry formation of human breast cancer cells
投稿时间:2010-09-09  
DOI:10.7655
中文关键词: 细胞凋亡  人乳腺癌细胞(MDA-MB-231)  拟态血管
英文关键词: apoptosis  human breast cancer cells(MDA-MB-231)  vasculogenic mimicry
基金项目:江苏省高校自然科学研究计划(07KJD310138);南京医科大学基础医学院青年教师培养基金(2010);江苏省卫生职业技术教育课题(J200501);江苏省高等学校大学生实践创新训练计划
作者单位
李卫星 泰州职业技术学院医学技术学院,江苏 泰州 225300 
孙重期 南京医科大学康达学院临床医学系06级,江苏 南京 210029 
杜 军 南京医科大学基础医学院生理学系,江苏 南京 210029 
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中文摘要:
      目的: 研究细胞凋亡对人乳腺癌细胞(MDA-MB-231)体外形成拟态血管的影响?方法:建立体外人工基底膜三维细胞培养模型,观察乳腺癌细胞能否形成血管样结构?用Annexin-FITC细胞凋亡检测试剂盒测定血管样结构形成过程中细胞凋亡情况;加入caspase抑制剂Z-VAD-FMK和caspase-3抑制剂Ac-DEVD-CHO后观察其对血管样结构的影响?结果:三维培养24 h时,乳腺癌细胞伸出细长突起,彼此相互连接,形成腔隙样结构;经PAS染色,可见环状PAS阳性图案,表明乳腺癌细胞在体外可以形成拟态血管?细胞三维培养后凋亡细胞数开始增多,8 h达到高峰,此后凋亡细胞数下降,24 h时凋亡细胞数与对照组相比无明显差异?Z-VAD-FMK和Ac-DEVD-CHO均能明显抑制三维培养状态下细胞拟态血管的形成?结论:在体外三维培养条件下,人乳腺癌细胞可形成拟态血管,而细胞凋亡过程是恶性肿瘤细胞拟态血管形成的关键环节之一?
英文摘要:
      Objective: To investigate the effect of cell apoptosis on the vasculogenic mimicry in human breast cancer cells(MDA-MB-231). Methods:The tube formation ability was assessed using a matrigel basement membrane model, and the structures of the tubular networks were observed under the inverted microscope. Apoptosis cells were stained with Annexin-FITC and PI,and then quantified by fluorescent microscope. Using the broad range caspase inhibitor Z-VAD-FMK and the caspase-3 inhibitor Ac-DEVD-CHO,we further explored the influence of cell apoptosis on forming capillary like structures. Results:MDA-MB-231 cells linked to each other to form wreaths and network structures after 24 h based on three dimensional culture. Capillary-like structures of MDA-MB-231 cells were stained positively with PAS. Apoptosis occurred before capillary-like structure formation but not after capillary-like structures had assembled. Both Z-VAD-FMK and Ac-DEVD-CHO blocked capillary-like structure formation. Conclusion:Breast cancer cells can adopt a capillary-like structure and engage in vasculogenic mimicry. Capillary-like structure formation requires apoptotic cell death through activation of a caspase-dependent mechanism.
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