文章摘要
赵旺胜,刘根焰,糜祖煌.产CTX-M-55-like型β-内酰胺酶同时伴膜孔蛋白ompC基因新突变等多株大肠埃希菌新菌株的发现[J].南京医科大学学报,2011,(2):203~209
产CTX-M-55-like型β-内酰胺酶同时伴膜孔蛋白ompC基因新突变等多株大肠埃希菌新菌株的发现
Discovery of new Escherichia coli strains containing new mutation types in ompC gene for outer membrane porin protein C combined with co-existing CTX-M-55-like beta-lactamase gene
投稿时间:2010-07-16  
DOI:10.7655
中文关键词: 大肠埃希菌  β-内酰胺酶基因  膜孔蛋白C  突变
英文关键词: Escherichia coli  beta-lactamase gene  outer membrane porin protein C  mutation
基金项目:江苏省实验诊断学重点实验室重大项目(XK200731)
作者单位
赵旺胜 南京医科大学第一附属医院检验科,江苏 南京 210029 
刘根焰 南京医科大学第一附属医院检验科,江苏 南京 210029 
糜祖煌 无锡市克隆遗传技术研究所生物信息学室,江苏 无锡 214026 
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中文摘要:
      目的: 调查大肠埃希菌(Escherichia coli,ECO)尿液分离株中β-内酰胺酶和膜孔蛋白ompC基因的存在和变异情况?方法: 收集南京医科大学第一附属医院2006年1月~2008年10月患者尿液标本中分离到的ECO菌共60株,采用聚合酶链反应(PCR)及序列分析的方法分析21种β-内酰胺酶基因和膜孔蛋白ompC基因?结果:在60株ECO菌中,TEM基因阳性51株(85.0%),经测序比对均为TEM-1-like;OXA-1群基因阳性6株(10.0%),经测序比对均为OXA-30-like;CTX-M-1群基因阳性38株(63.3%),经测序比对均为CTX-M-55-like?膜孔蛋白ompC基因经测序比对60株中有6株(10.0%)无突变,其余54株均有突变(突变率90.0%),其中6株经BLASTn比对确认为新的突变类型?结论:产CTX-M-55-like型β-内酰胺酶和膜孔蛋白ompC基因是本组ECO菌对β-酰胺类抗菌药物产生耐药的重要原因?6株ompC基因新的突变类型中,3株为同时携带TEM-1-like?CTX-M-55-like基因的新菌株;2株为携带了TEM-1-like基因的新菌株;1株为同时携带了TEM-1-like?OXA-30-like基因的新菌株?
英文摘要:
      Objective: To investigate the distribution and variety of beta-lactamase genes and ompC gene for outer membrane porin protein C in Escherichia coli (E.coli) strains isolated from urine. Methods:From January 2006 to October 2008, 60 strains of E.coli isolated from urine were collected from The First Affiliated Hospital of NJMU, China. Then, 21 kinds of beta-lactamase genes and ompC gene for outer membrane porin protein C were analyzed by PCR and verified by DNA sequencing. Results: In 60 strains of E.coli, 51 strains (85.0%) , 6 strains(10.0%)and 38 strains(63.3%) were detected to harbor TEM-1-like,OXA-30-like and CTX-M-55-like by DNA sequencing, respectively. Moreover, 54 strains were detected mutaions in ompC gene for outer membrane porin protein C (90.0%), and 6 strains among them were confirmed as new mutation types by BLASTn. But other 6 strains(10.0%) were detected no mutaion in ompC gene. Conclusion:Both CTX-M-55-like beta-lactamase gene and ompC gene for outer membrane porin protein C play a key role in resistance to beta-lactam antimicrobial agents. Among 6 strains of E.coli containing new mutation types in ompC gene for outer membrane porin protein C, 3 strains combined with co-existing TEM-1-like and CTX-M-55-like beta-lactamase genes, 2 strains combined with co-existing TEM-1-like beta-lactamase gene, 1 strain combined with co-existing TEM-1-like and OXA-30-like beta-lactamase genes.
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