文章摘要
钱涛涛,张石江.肿瘤坏死因子-β rs1041981位点基因多态性与冠心病遗传易感性关系[J].南京医科大学学报,2011,(3):339~342
肿瘤坏死因子-β rs1041981位点基因多态性与冠心病遗传易感性关系
The relationship between tumor necrosis factor-β rs1041981 gene polymorp- hisms and coronary artery disease in the patients of nanjing area
投稿时间:2010-09-19  
DOI:10.7655
中文关键词: 肿瘤坏死因子-β  冠状动脉粥样硬化性心脏病  taqman  基因多态性
英文关键词: tumor necrosis factor-β  coronary artery disease  taqman  gene polymorphism
基金项目:
作者单位
钱涛涛 南京医科大学第一附属医院胸心外科 ,江苏 南京 210029 
张石江 南京医科大学第一附属医院胸心外科 ,江苏 南京 210029 
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中文摘要:
      目的:探讨肿瘤坏死因子-β(tumor necrosis factor-β, tnf-β)rs1041981位点基因多态性与南京地区老年冠状动脉粥样硬化性心脏病(coronary artery disease,cad)遗传易感性的关系?方法:采用病例对照研究方法,选择确诊的399例cad患者和280例健康对照者,应用高通量taqman-mgb探针技术对tnf-β rs1041981位点进行基因分型,同时采用荧光定量pcr法对其进行检测?结果:cad组tnf-β rs1041981基因型分布(cc型25.1%?ca型50.3%?aa型24.6%)与对照组(41.1%?41.8%?17.1%)的差异具有统计学意义(χ2=20.1,p < 0.05);基因型频率的相对风险分析发现,aa型及ca型患cad的风险是cc型的1.38倍(or=1.38,95%ci:0.84~2.05);cad组tnf-β及超敏c反应蛋白血清水平[(38.74±16.42)pg/ml,(6.22±3.74)mg/l]显著高于对照组[(12.26±10.37)pg/ml,(2.37±1.17)mg/l,p < 0.05]?结论:tnf-β rs1041981基因多态性与本地区cad的发病有关,等位基因a可能是cad发生的重要易感基因?
英文摘要:
      objective:to investigate the relationship between tumor necrosis factor-β(tnf-β) rs1041981 gene polymorphisms and coronary artery disease(cad) in nanjing city. methods:a case-control study comprising 399 patients with cad and 280 controls was performed. single nucleotide polymorphisms of rs1041981 in tnf-β gene were genotyped and analyzed using the high-flux taqman probe technology coupled with fluorescent quantitative pcr method. results:the frequencies of the cc, ca, and aa genotypes of rs1041981 were 25.1%, 50.3% and 24.6% in cad group and were 41.1%,41.8% and 17.1% in control group respectively. there were statistically significant differences in the distributions of the genotypes (χ2=20.1,p < 0.05). the risk of cad in those of ca and aa genotypes was 1.38 times of cc genotype(or=1.38,95%ci:0.84~2.05). the serum tnf-β and high sensitive c-reactive protein levels of the cad group were significantly higher than that of the control group(p < 0.05).conclusion:the single nucleotide polymorphism (snp) of rs1041981 in tnf-β gene is associated with cad in nanjing area and the allele a may be a risk factor of cad.
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