文章摘要
鲁 严,周梅华,吴 迪,王大光,朱 丰.过氧化氢诱导条件下人黑素细胞树突变化及其胞内Ca2+浓度变化的研究[J].南京医科大学学报,2011,(3):360~363
过氧化氢诱导条件下人黑素细胞树突变化及其胞内Ca2+浓度变化的研究
Study on morphological alterations of dendrites and intracellular calcium concentration of human melanocytes induced by hydrogen perioxide
投稿时间:2010-09-23  
DOI:10.7655
中文关键词: 过氧化氢  黑素细胞  树突  钙离子
英文关键词: hydrogen peroxide  melanocyte  dendrite  calcium
基金项目:
作者单位
鲁 严 南京医科大学第一附属医院皮肤科,江苏 南京 210029 
周梅华 南京医科大学第一附属医院皮肤科,江苏 南京 210029 
吴 迪 南京医科大学第一附属医院皮肤科,江苏 南京 210029 
王大光 南京医科大学第一附属医院皮肤科,江苏 南京 210029 
朱 丰 南京医科大学第一附属医院皮肤科,江苏 南京 210029 
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中文摘要:
      目的:通过体外过氧化氢诱导模拟体内氧化压力条件下,联合观察人黑素细胞树突变化及其细胞内Ca2+浓度变化,初步说明黑素细胞经表皮丢失病理生理学基础?方法:采用常规分离培养人黑素细胞,分别用终浓度为0.1%和0.05%的H2O2作用于黑素细胞培养基中,于30?60?90?120和150 min后观察黑素细胞的树突变化,并通过目镜标尺测定树突变化率?另用Flu-3/AM染色,流式细胞术测定黑素细胞内钙离子荧光强度值?结果:使用不同浓度的H2O2诱导黑素细胞后树突显著回缩,0.1%H2O2对树突缩短作用强于0.05%H2O2;细胞内钙离子的荧光探针检测显示黑素细胞经过H2O2诱导后细胞内钙离子浓度升高,并且钙离子出现的峰值在诱导30 min时,早于黑素细胞树突缩短变化第1高峰60 min节点?结论:黑素细胞树突缩短可能与细胞内钙离子堆积,不能发生钙内流有关?
英文摘要:
      Objective: To elucidate pathophysiology of melanocytorrhagy by investigating morphological alterations of dendrites and intracellular calcium concentration(Ca2+) of human melanocytes induced by hydrogen perioxide(H2O2) in vitro. Methods:Melanocytes were cultured routinely, and 0.1% and 0.05% H2O2 were added to culture medium respectively. Dendrites’ length was observed at 30,60,90,120 and 150 min after treatment,and measured accurately by microscope optical ruler. Intracellular calcium was detected with Flu-3/AM probe by flow cytometry. Results:H2O2 induced a notable retraction of dendrites,of which concentration 0.1% had more potent effect than 0.05%. Intracellular calcium was up-regulated with the inducement of H2O2 detected by Flu-3/AM probe. Calcium peak presented was at 30 min after treatment, which is earlier than the first retraction peak of dendrites at 60 min. Conclusion:The results suggest retraction of dendrites might be related to accumulation of intracellular calcium, which led to failure of reflux of calcium.
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