文章摘要
李倩倩,蒋秀琴,李慧敏,缪 珩.2型糖尿病患者皮下?大网膜脂肪组织脂联素mRNA表达变化及意义[J].南京医科大学学报,2011,(3):404~409
2型糖尿病患者皮下?大网膜脂肪组织脂联素mRNA表达变化及意义
Expression and significance of adiponectin mRNA in subcutaneous and omental adipose tissue of type 2 diabetic patients
投稿时间:2010-09-17  
DOI:10.7655
中文关键词: 脂联素  2型糖尿病  体质指数  胰岛素抵抗
英文关键词: adiponectin  type 2 diabetes mellitus  body mass index  insulin resistance
基金项目:南京医科大学重点课题基金资助(07NMUZ014)
作者单位
李倩倩 南京医科大学第二附属医院内分泌科,江苏 南京 210011 
蒋秀琴 南京医科大学第二附属医院内分泌科,江苏 南京 210011 
李慧敏 南京医科大学第二附属医院内分泌科,江苏 南京 210011 
缪 珩 南京医科大学第二附属医院内分泌科,江苏 南京 210011 
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中文摘要:
      目的:观察2型糖尿病患者皮下及大网膜脂肪组织脂联素(adiponectin)表达水平的变化,及其与血清脂联素?体质指数(BMI)?腰围(WC),腰/臀比值(WHR)?收缩压(SBP)?舒张压(DBP)?空腹血糖(FPG)?糖化血红蛋白(HbA1c)?总胆固醇(TC)?甘油三酯(TG)?低密度脂蛋白胆固醇(LDL-C)?高密度脂蛋白胆固醇(HDL-C)?尿酸(UA)等相关指标的关系,探讨脂联素在2型糖尿病患者发生胰岛素抵抗中的作用?方法:用半定量RT-PCR方法检测23例2型糖尿病合并肥胖患者(DM+FAT)?23例2型糖尿病患者(DM)?33例非糖尿病肥胖患者(control+FAT)?23例非糖尿病患者(control)腹部皮下及大网膜脂肪组织脂联素mRNA的表达水平,并测量身高?体重?腰围?臀围?血压?FPG?HbA1C?血脂?尿酸?血清脂联素,并计算BMI?WHR?结果:①糖尿病肥胖组SBP?DBP?FPG?HbA1c?TG?LDL-C?尿酸均高于对照肥胖组,HDL-C?皮下及大网膜脂肪组织脂联素mRNA表达量?血清脂联素均低于对照肥胖组(P < 0.01)?两组的大网膜脂联素表达量低于其皮下表达量(P < 0.01);②糖尿病非肥胖组的FPG?HbA1c?TG?尿酸均高于对照非肥胖组,HDL-C?皮下及大网膜脂肪组织脂联素mRNA表达量?血清脂联素均低于对照非肥胖组(P < 0.01)?两组的大网膜脂联素表达量低于其皮下表达量(P < 0.01);③糖尿病肥胖组的BMI?WHR?SBP?DBP?FPG?HbA1c?TG?尿酸均高于糖尿病非肥胖组,大网膜脂肪组织脂联素mRNA表达量?血清脂联素均低于糖尿病非肥胖组(P < 0.01);④直线相关分析显示,糖尿病肥胖组的网膜脂联素mRNA的表达水平与BMI?FPG(P < 0.01)?WHR?WC(P < 0.05)呈负相关,与血清脂联素水平呈正相关(P < 0.01)?结论:2型糖尿病合并肥胖患者大网膜脂肪组织脂联素mRNA表达显著降低,血清脂联素水平显著降低;BMI?WC?WHR?FPG等临床指标可间接反映胰岛素抵抗程度?
英文摘要:
      Objective: To observe the adiponectin mRNA expression in subcutaneous and omental adipose tissue of type 2 diabetic patients and their relation with serum adiponectin, body mass index(BMI), waist circumference(WC),waist hip ratio(WHR), blood pressure, fasting plasma glucose(FPG), HbA1c, blood lipids and uric acid(UA). Methods:Twenty-three T2DM patients with obesity, 23 T2DM patients, 33 obesity patients and 23 controls were selected. The adiponectin mRNA expression level in adipose tissue was assayed by RT-PCR. The level of serum adiponectin was measured with ELISA kit. FBG, triglyceride(TG), total cholesterol(TC), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), HbA1c, UA of all subjects were determined and their blood pressure, height, weight, waist circumference, hip circumference were measured to calculate BMI,WHR. Results:①The SBP, DBP, FPG, HbA1c, TG, LDL-C, UA were significantly higher, and serum adiponectin, HDL-C and adiponectin mRNA expression level in both subcutaneous and omental adipose tissue were significantly lower in diabetic obesity group than in obesity group(P < 0.05), the adiponectin mRNA expression level in omental were significantly lower than in subcutaneous adipose tissue in both groups(P < 0.01). ②The FPG, HbA1c, TG, UA were higher, serum adiponectin, HDL-C and adiponectin mRNA levels expressed in both subcutaneous and omental adipose tissue were lower in diabetic group without obesity than in control group without obesity(P < 0.05), the adiponectin mRNA expression level in omental was lower than in subcutaneous adipose tissue in both groups(P < 0.01). ③The BMI,WHR,SBP,DBP,FPG,HbA1C,TG,UA were higher, serum adiponectin and adiponectin mRNA level expressed in both subcutaneous and omental adipose tissue were lower in diabetic obese group than in diabetic group without obesity(P < 0.05). ④The adiponectin mRNA expression level in omental adipose tissue of diabetic obesity group was negatively correlated to BMI,WHR,FPG(P < 0.05), and was positively correlated to serum adiponectin(P < 0.01). Conclusion:T2DM patients with obesity show down-regulated expression of adiponectin mRNA in omental adipose tissue and lower serum adiponectin than control obese group,BMI,WC,WHR,FPG are correlated with adiponectin mRNA expression and insulin resistance.
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