Objective:To observe the effects of Shanggan Granule on the express of Toll-like receptor 3(TLR3) and double-stranded RNA-dependent protein kinase(PKR) mRNA in lung of mice with viral upper respiratory tract infection,and explore its immune regulation mechanism. Methods:ICR mice were randomly divided into normal group,model group,antiviral oral liquid group,ribavirin group,Shanggan Granule high-dose group,Shanggan Granule medium-dose group and Shanggan Granule low-dose group.Mice were infected with influenza A virus (A/PR8/34) venom intranasally and treated by different way,the expressions of TLR3 and PKR mRNA were detected by RT-PCR. Results:Compared with the model group,the levels of TLR3 and PKR mRNA in antiviral oral liquid group,ribavirin group and Shanggan Granule groups were reduced significantly(P < 0.05). Conclusion:Shanggan Granule can reduce the expressions of TLR3 and PKR mRNA in lung of mice with viral upper respiratory tract infection,and this way contribute to the immune regulation of Shanggan Granule.