文章摘要
郑艳,耿连艺,盛夏,高峰,奚肇庆.上感颗粒对病毒性上呼吸道感染小鼠肺组织TLR3及PKR mRNA表达的影响[J].南京医科大学学报,2011,(3):410~413
上感颗粒对病毒性上呼吸道感染小鼠肺组织TLR3及PKR mRNA表达的影响
Effect of Shanggan Granule on the express of TLR3 and PKR mRNA in lung of mice with viral upper respiratory tract infection
投稿时间:2010-07-30  
DOI:10.7655
中文关键词: 上感颗粒  病毒性上呼吸道感染  TLR3  PKR  mRNA
英文关键词: Shanggan Granule  viral upper respiratory tract infection  TLR3  PKR  mRNA
基金项目:2010南京中医药大学大学生实践创新训练计划;2009江苏省教育厅研究生科研创新计划(CX09B-2007Z);国家中医药管理局08行业科研专项(200807041)
作者单位
郑艳 南京中医药大学第一临床医学院内科学教研室,江苏 南京 210046 
耿连艺 南京中医药大学第一临床医学院内科学教研室,江苏 南京 210046 
盛夏 南京中医药大学第一临床医学院内科学教研室,江苏 南京 210046 
高峰 东南大学医学院分子生物学实验室,江苏 南京210009 
奚肇庆 江苏省中医院急诊科,江苏 南京 210029 
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中文摘要:
      目的:通过观察上感颗粒对病毒性上呼吸道感染小鼠肺组织TLR3及PKR mRNA表达的影响,来探讨其治疗病毒性上呼吸道感染可能的免疫调节机制?方法:ICR小鼠随机分为7组:正常对照组?模型组?抗病毒口服液组?利巴韦林组?上感颗粒大?中?低剂量组?病毒滴鼻后给药,观察其肺组织TLR3及PKR mRNA的表达?结果:抗病毒口服液?利巴韦林?上感颗粒可降低小鼠肺组织TLR3及PKR mRNA的表达,与模型组比较具有显著性差异(P < 0.05)?结论:上感颗粒可抑制病毒性上呼吸道感染小鼠肺组织中TLR3及PKR mRNA的表达,可能为其免疫调节作用的机制之一?
英文摘要:
      Objective:To observe the effects of Shanggan Granule on the express of Toll-like receptor 3(TLR3) and double-stranded RNA-dependent protein kinase(PKR) mRNA in lung of mice with viral upper respiratory tract infection,and explore its immune regulation mechanism. Methods:ICR mice were randomly divided into normal group,model group,antiviral oral liquid group,ribavirin group,Shanggan Granule high-dose group,Shanggan Granule medium-dose group and Shanggan Granule low-dose group.Mice were infected with influenza A virus (A/PR8/34) venom intranasally and treated by different way,the expressions of TLR3 and PKR mRNA were detected by RT-PCR. Results:Compared with the model group,the levels of TLR3 and PKR mRNA in antiviral oral liquid group,ribavirin group and Shanggan Granule groups were reduced significantly(P < 0.05). Conclusion:Shanggan Granule can reduce the expressions of TLR3 and PKR mRNA in lung of mice with viral upper respiratory tract infection,and this way contribute to the immune regulation of Shanggan Granule.
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